Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/14970

Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners

Title: Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners
Authors : Fernández Sanjurjo, Manuel
Úbeda Martín, Natalia
Fernández García, Benjamín
Valle, Miguel del
Ramírez de Molina, Ana
Ramírez de Molina, Ana
Crespo, María Carmen
Martín Hernández, Roberto
Casas Agustench, Patricia
Martínez Clambor, Pablo
Gonzalo Calvo, David de
Díez Robles, Sergio
García González, Ángela
Montero, Ana
González González, Felipe
Rabadán, Manuel
Díaz Martínez, Ángel Enrique
Whitham, Martin
Iglesias Gutiérrez, Eduardo
Dávalos, Alberto
Keywords: Circulating microRNAsEndurance exerciseExercise biomarkersExercise dose
Publisher: Wiley
Citation: Fernández-Sanjurjo M, Úbeda N, Fernández-García B, et al. Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners. Scand J Med Sci Sports. 2020;30:1896–1907.
Abstract: The systemic response to exercise is dose-dependent and involves a complex gene expression regulation and cross-talk between tissues. This context ARISES the need for analyzing the influence of exercise dose on the profile of circulating microRNAs (c-miRNAs), as emerging posttranscriptional regulators and intercellular communicators. Thus, we hypothesized that different exercise doses will determine specific c-miRNA signatures that will highlight its potential as exercise dose biomarker. Nine active middle-aged males completed a 10-km race (10K), a half-marathon (HM), and a marathon (M). Blood samples were collected immediately before and after races. Plasma RNA was extracted, and a global screening of 752 microRNAs was analyzed using RT-qPCR. Three different c-miRNA profiles were defined according to the three doses. In 10K, 14 c-miRNAs were found to be differentially expressed between pre- and post-exercise, 13 upregulated and 1 downregulated. Regarding HM, 13 c-miRNAs were found to be differentially modulated, in all the cases upregulated. A total of 28 c-miRNAs were found to be differentially expressed in M, 21 overexpressed and 7 repressed after this race. We had also found 3 common c-miRNAs between 10K and M and 2 common c-miRNAs between 10K and HM. In silico analysis supported a close association between exercise dose c-miRNA profiles and cellular pathways linked to energy metabolism and cell cycle. In conclusion, we have observed that different exercise doses induced specific c-miRNA profiles. So, our results point to c-miRNAs as emerging exercise dose biomarkers and as one of regulatory mechanisms modulating the response to endurance exercise.
URI: http://hdl.handle.net/10637/14970
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
OpenAccess
ISSN: 1600-0838
Issue Date: 23-Jul-2020
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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