Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/14706

Physiological and Genetic Modifications Induced by Plant-Growth-Promoting Rhizobacteria (PGPR) in Tomato Plants under Moderate Water Stress

Title: Physiological and Genetic Modifications Induced by Plant-Growth-Promoting Rhizobacteria (PGPR) in Tomato Plants under Moderate Water Stress
Authors : Lucas García, José Antonio
García Villaraco, Ana
Montero Palmero, María Belén
Montalbán Ginés, Blanca
Ramos Solano, Beatriz
Gutierrez-Mañero, Francisco Javier
Keywords: Water stressPGPRPEG6000Oxidative stressPhotosynthesis efficiencyP5CSNCDE1Plasma membrane ATPase
Publisher: MDPI
Citation: Lucas, J.A.; Garcia-Villaraco, A.; Montero-Palmero, M.B.; Montalban, B.; Ramos Solano, B.; Gutierrez-Mañero, F.J. Physiological and Genetic Modifications Induced by Plant-Growth-Promoting Rhizobacteria (PGPR) in Tomato Plants under ModerateWater Stress. Biology 2023, 12, 901. https:// doi.org/10.3390/biology12070901
Abstract: Physiological, metabolic, and genetic changes produced by two plant growth promoting rhizobacteria (PGPR) Pseudomonas sp. (internal code of the laboratory: N 5.12 and N 21.24) inoculated in tomato plants subjected to moderate water stress (10% polyethylene glycol-6000; PEG) were studied. Photosynthesis efficiency, photosynthetic pigments, compatible osmolytes, reactive oxygen species (ROS) scavenging enzymes activities, oxidative stress level and expression of genes related to abscisic acid synthesis (ABA; 9-cis-epoxycarotenoid dioxygenase NCDE1 gene), proline synthesis (Pyrroline-5-carboxylate synthase P5CS gene), and plasma membrane ATPase (PM ATPase gene) were measured. Photosynthetic efficiency was compromised by PEG, but bacterial-inoculated plants reversed the effects: while N5.12 increased carbon fixation (37.5%) maintaining transpiration, N21.24 increased both (14.2% and 31%), negatively affecting stomatal closure, despite the enhanced expression of NCDE1 and plasma membrane ATPase genes, evidencing the activation of different adaptive mechanisms. Among all parameters evaluated, photosynthetic pigments and antioxidant enzymes guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) responded differently to both strains. N 5.12 increased photosynthetic pigments (70% chlorophyll a, 69% chlorophyll b, and 65% carotenoids), proline (33%), glycine betaine (4.3%), and phenolic compounds (21.5%) to a greater extent, thereby decreasing oxidative stress (12.5% in Malondialdehyde, MDA). Both bacteria have highly beneficial effects on tomato plants subjected to moderate water stress, improving their physiological state. The use of these bacteria in agricultural production systems could reduce the amount of water for agricultural irrigation without having a negative impact on food production.
URI: http://hdl.handle.net/10637/14706
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Open Access
ISSN: 2079-7737
Issue Date: 23-Jun-2023
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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