Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/14500

Impact of caloric restriction on AMPK and endoplasmic reticulum stress in peripheral tissues and circulating peripheral blood mononuclear cells from Zucker rats

Title: Impact of caloric restriction on AMPK and endoplasmic reticulum stress in peripheral tissues and circulating peripheral blood mononuclear cells from Zucker rats
Authors : Vega Martín, Elena
González Blázquez, Raquel
Manzano Lista, Francisco J.
Martín Ramos, Miriam
García Prieto, Concepción F.
Viana Arribas, Marta
Rubio, Miguel A.
Calle Pascual, Alfonso L.
Lionetti, Lillà
Somoza Hernández, Beatriz
Fernández Alfonso, María Soledad
Alcalá Díaz-Mor, Martín
Gil Ortega, Marta
Keywords: Caloric restrictionEndoplasmic reticulum stressObesityCirculating peripheral blood mononuclear cells
Publisher: Elsevier
Citation: Vega-Martín E, González-Blázquez R, Manzano-Lista FJ, Martín-Ramos M, García-Prieto CF, Viana M, Rubio MA, Calle-Pascual AL, Lionetti L, Somoza B, Fernández-Alfonso MS, Alcalá M, Gil-Ortega M. Impact of caloric restriction on AMPK and endoplasmic reticulum stress in peripheral tissues and circulating peripheral blood mononuclear cells from Zucker rats. J Nutr Biochem. 2020 Apr;78:108342. doi: 10.1016/j.jnutbio.2020.108342. Epub 2020 Jan 9.
Abstract: The activation of endoplasmic reticulum (ER) stress and a reduction of AMP-dependent protein kinase (AMPK) phosphorylation have been described in obesity. We hypothesize that a moderate caloric restriction (CR) might contribute to reducing ER stress and increasing AMPK phosphorylation in peripheral tissues from genetically obese Zucker fa/fa rats and in peripheral blood mononuclear cells (PBMCs). Zucker Lean and Zucker fa/fa rats were fed with chow diet either ad libitum (AL) (C, as controls) or 80% of AL (CR) for 2 weeks, giving rise to four experimental groups: Lean C, Lean CR, fa/fa C and fa/fa CR. CR significantly increased AMPK phosphorylation in the liver, perirenal adipose tissue (PRAT) and PBMCs from fa/fa rats but not in the subcutaneous AT (SCAT), suggesting a reduced response of SCAT to CR. Liver samples of fa/fa rats exhibited an increased mRNA expression of PERK, EIF-2α, XBP-1(s), Chop and caspase 3, which was significantly reduced by CR. PRAT exhibited an overexpression of Edem and PDIA-4 in fa/fa rats, but only PDIA-4 expression was reduced by CR. eIF-2α phosphorylationwas significantly increased in all studied tissues fromfa/fa rats and reduced by CR. A negative correlationwas detected between p-AMPK and p-eIF-2α in the liver, PRAT and PBMCs from fa/fa rats but not in SCAT. This study shows that a moderate CR reduces ER stress and improves AMPK phosphorylation in several peripheral tissues and in circulating PBMCs, suggesting that alterations observed in PBMCs could reflect metabolic alterations associated with obesity. © 2020 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/10637/14500
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
openAccess
Issue Date: 28-Jan-2020
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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