Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/14116

Deciphering the role of platelets in severe allergy by an integrative omics approach.

Title: Deciphering the role of platelets in severe allergy by an integrative omics approach.
Descifrando el papel de las plaquetas en la alergia severa mediante un enfoque ómico integrador
Authors : Pablo-Torres, Carmela
Izquierdo Álvarez, Elena
Ju Tan, Tiak
Obeso Montero, David
Layhadi, Janice A.
Sánchez-Solares, Javier
Mera Berriatua, Leticia
Bueno-Cabrera, José Luis
Reaño-Martos, María del Mar
Iglesias-Cadarso, Alfredo
Barbas Arribas, Coral.
Gómez Casado, Cristina
Villaseñor Solis, Alma Cristina
Barber Hernández, Domingo
H. Shamji, Mohamed
Escribese Alonso, María Marta
Keywords: AllergylipidomicsmetabolomicsplateletsRNAseq
Publisher: Wiley
Citation: Pablo-Torres C, Izquierdo E, Tan TJ, et al. Deciphering the role of platelets in severe allergy by an integrative omics approach. Allergy. 2023;00:1-14. doi:10.1111/all.15621
Abstract: Background: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction. Methods: Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) samples were obtained by platelet-apheresis from severe (n = 7) and mild (n = 10) allergic patients and nonallergic subjects (n = 9) to perform platelet lipidomics by liquid chromatography coupled to mass spectrometry (LC–MS) and RNA-seq analysis. Significant metabolites and transcripts were used to identify compromised biological pathways in the severe phenotype. Platelet and inflammation-related proteins were quantified by Luminex. Results: Platelets from severe allergic patients were characterized by high levels of ceramides, phosphoinositols, phosphocholines, and sphingomyelins. In contrast, they showed a decrease in eicosanoid precursor levels. Biological pathway analysis performed with the significant lipids revealed the alteration of phospholipases, calcium-dependent events, and linolenic metabolism. RNAseq confirmed mRNA overexpression of genes related to platelet activation and arachidonic acid metabolism in the severe phenotypes. Pathway analysis indicated the alteration of NOD, MAPK, TLR, TNF, and IL-17 pathways in the severe phenotype. P-Selectin and IL-17AF proteins were increased in the severe phenotype. Conclusions: This study demonstrates that platelet lipid, mRNA, and protein content is different according to allergy severity. These findings suggest that platelet load is a potential source of biomarkers and a new chance for therapeutic targets in severe inflammatory pathologies.
URI: http://hdl.handle.net/10637/14116
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
openAccess
ISSN: 1398-9995 (Electrónico)
Supported by: Acuerdo Transformativo - 2023
Issue Date: 17-Dec-2022
Center : Universidad San Pablo-CEU
Appears in Collections:Medicina





Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.