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dc.contributor.otherUniversidad San Pablo-CEU. Facultad de Medicina-
dc.creatorPablo-Torres, Carmela-
dc.creatorIzquierdo Álvarez, Elena-
dc.creatorJu Tan, Tiak-
dc.creatorObeso Montero, David-
dc.creatorLayhadi, Janice A.-
dc.creatorSánchez-Solares, Javier-
dc.creatorMera Berriatua, Leticia-
dc.creatorBueno-Cabrera, José Luis-
dc.creatorReaño-Martos, María del Mar-
dc.creatorIglesias-Cadarso, Alfredo-
dc.creatorBarbas Arribas, Coral.-
dc.creatorGómez Casado, Cristina-
dc.creatorVillaseñor Solis, Alma Cristina-
dc.creatorBarber Hernández, Domingo-
dc.creatorH. Shamji, Mohamed-
dc.creatorEscribese Alonso, María Marta-
dc.date2022-
dc.date.accessioned2023-02-07T05:00:16Z-
dc.date.available2023-02-07T05:00:16Z-
dc.date.issued2022-12-17-
dc.identifier000000735843-
dc.identifier.citationPablo-Torres C, Izquierdo E, Tan TJ, et al. Deciphering the role of platelets in severe allergy by an integrative omics approach. Allergy. 2023;00:1-14. doi:10.1111/all.15621-
dc.identifier.issn1398-9995 (Electrónico)-
dc.identifier.urihttp://hdl.handle.net/10637/14116-
dc.description.abstractBackground: Mechanisms causing the onset and perpetuation of inflammation in severe allergic patients remain unknown. Our previous studies suggested that severe allergic inflammation is linked to platelet dysfunction. Methods: Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) samples were obtained by platelet-apheresis from severe (n = 7) and mild (n = 10) allergic patients and nonallergic subjects (n = 9) to perform platelet lipidomics by liquid chromatography coupled to mass spectrometry (LC–MS) and RNA-seq analysis. Significant metabolites and transcripts were used to identify compromised biological pathways in the severe phenotype. Platelet and inflammation-related proteins were quantified by Luminex. Results: Platelets from severe allergic patients were characterized by high levels of ceramides, phosphoinositols, phosphocholines, and sphingomyelins. In contrast, they showed a decrease in eicosanoid precursor levels. Biological pathway analysis performed with the significant lipids revealed the alteration of phospholipases, calcium-dependent events, and linolenic metabolism. RNAseq confirmed mRNA overexpression of genes related to platelet activation and arachidonic acid metabolism in the severe phenotypes. Pathway analysis indicated the alteration of NOD, MAPK, TLR, TNF, and IL-17 pathways in the severe phenotype. P-Selectin and IL-17AF proteins were increased in the severe phenotype. Conclusions: This study demonstrates that platelet lipid, mRNA, and protein content is different according to allergy severity. These findings suggest that platelet load is a potential source of biomarkers and a new chance for therapeutic targets in severe inflammatory pathologies.en_EN
dc.description.sponsorshipAcuerdo Transformativo - 2023-
dc.formatapplication/pdf-
dc.language.isoen-
dc.publisherWiley-
dc.relation.ispartofAllergy-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.rightsopenAccess-
dc.subjectAllergyen_EN
dc.subjectlipidomicsen_EN
dc.subjectmetabolomicsen_EN
dc.subjectplateletsen_EN
dc.subjectRNAseqen_EN
dc.titleDeciphering the role of platelets in severe allergy by an integrative omics approach.en_EN
dc.titleDescifrando el papel de las plaquetas en la alergia severa mediante un enfoque ómico integradores_ES
dc.typeArtículo-
dc.identifier.doihttps://doi.org/10.1111/all.15621-
dc.relation.projectIDFundación Mutua Madrileña, Grant/Award Number: AP177712021; Instituto de Salud Carlos III, Grant/Award Number: PI18/01467,PI19/00044; Junta de Andalucía, Grant/Award Number: PC- 0278- 2017; Ministerio de Ciencia, Innovación y Universidades, Grant/Award Number: PCI2018-092930; Comunidad de Madrid; Instituto de Salud Carlos III- European Regional Development Fund, Grant/Award Number: RD16/0006/0015,RD21/0002/0008; Horizon 2020 Framework Programme; Ministerio de Ciencia, Innovación y Universidades, Grant/Award Number: PCI2018-092930; Universidad San Pablo-CEU-
dc.centroUniversidad San Pablo-CEU-
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