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http://hdl.handle.net/10637/1047A rapid and sensitive method for HPLC cholesterol determination in bile.
| Title: | A rapid and sensitive method for HPLC cholesterol determination in bile. |
| Authors : | Bocos de Prada, Carlos Castro, Mario Orozco, Enrique. Contreras, Juan Antonio Herrera Castillón, Emilio. |
| Keywords: | Determinación de colesterol |
| Abstract: | A relatively little time consuming simple method based on the treatment of bile
with cholesterol oxidase and subsequent high performance liquid chromatography
measurement of the 3-ketocholesterol produced in order to determine the level of
the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100 % values. It is highly
sensiti\·e and suitable for use in the determination of cholesterol concentrations in
bile and other bilirubin containing biological fluids. Se describe un rnétodo sencillo y rápido para determinar !as concentraciones de colesterol en bilis, basado en la formacion de 3-cetocolestcrol, mediante el tratamiento con colesterol oxidasa, y su mcdida por cromatografia de alta resolucion. El metodo evita !as interferencias con la bilirrubina y presenta una alta reproducibilidad. Los ensayos de recuperacion dan valores del I 00 % . El metodo es de alta sensibilidad y apropiado para cuantificar el colesterol en bilis y en otros fluidos biologicos que comengan bilirrubina. |
| Description: | En: Revista Española de Fisiología, ISSN 0034-9402 1992. N. 48, pp 211-224 |
| URI: | http://hdl.handle.net/10637/1047 |
| Rights : | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
| Issue Date: | 19-Sep-1992 |
| Center : | Universidad San Pablo-CEU |
| Appears in Collections: | Facultad de Farmacia |
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