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Separation and identification of organic acids in root exudates of lupinus luteus by capillary zone electrophoresis.
Title: | Separation and identification of organic acids in root exudates of lupinus luteus by capillary zone electrophoresis. |
Authors : | Barbas Arribas, Coral. Lucas García, José Antonio Gutiérrez Mañero, Francisco Javier |
Keywords: | Capillary electrophoresis; Organic acids; Exudates; Lupinus |
Abstract: | The separation and identification of a mixture of organic acids by capillary electrophoresis, with direct detection by UV absorption at a wavelength of 200 nm is reported. A solution containing the following organic acids was studied: oxalic, formic, fumaric, malic, succinic, citric, acetic and lactic acid. Separation was achieved using a fused-silica capillary filled with phosphate buffer (200 mM; pH 6) containing N-acetylN,N,N-trimethylammonium bromide (0.5 mM) as cationic surfactant, necessary to modify the electrosmotic flow. The composition of organic acids in exudates of Lupinus luteus was determined by this method. |
Description: | En: Phytochemical analysis. ISSN. 0958-0344. n. 10 (1999), p. 55-59 |
URI: | http://hdl.handle.net/10637/657 |
Rights : | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
Issue Date: | 19-Sep-1999 |
Center : | Universidad San Pablo-CEU |
Appears in Collections: | Facultad de Farmacia |
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