Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/15896

Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: cell, and PDGF-BB and TGF-ß1 growth factor analysis

Title: Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: cell, and PDGF-BB and TGF-ß1 growth factor analysis
Authors : Miguel Pastor, Laura
Satué Ambrojo, Katiuska
Chicharro Alcántara, Deborah
Damiá Giménez, Elena
Cuervo Serrato, Belén
Torres Torrillas, Marta
Martins, Emma Julie Camille
Velasco Martínez, María Gemma
Carrillo Poveda, José María
Sopena Juncosa, Joaquín Jesús
Cerón, José J.
Rubio Zaragoza, Mónica
Keywords: Plasma sanguíneoBlood plasmaPlaquetasBlood plateletsFactores de crecimientoGrowth factorsGatoCatsEnfermedad animalAnimal diseases
Publisher: Elsevier
Citation: Miguel-Pastor, L., Satué, K., Chicharro, D., Damiá, E., Cuervo, B., Torres-Torrillas, M., Martins, E., Velasco-Martínez, M.G., Carrillo, J.M., Sopena, J.J., Cerón, J.J. & Rubio, M. (2024). Characterization of platelet rich plasma in feline immunodeficiency virus-infected cats: cell, and PDGF-BB and TGF-ß1 growth factor analysis. Research in Veterinary Science, vol. 168 (mar.), art. 105138. DOI: https://doi.org/10.1016/j.rvsc.2024.105138
Abstract: Autologous platelet-rich plasma (PRP) contains growth factors (GFs) that modulate the expression of inflammatory cells; thus, these products could be considered a good strategy to favor tissue regeneration in feline immunodeficiency (FIV) positive cats. However, there is no scientific documentation on obtaining PRP in FIV-positive cats. Authors hypothesized that PRP can be obtained in FIV cats following the PRGF®-Endoret® methodology. The objectives of this study were to compare the platelet, erythrocyte, and leukocyte concentration between whole blood (WB) and the PRP; and determine the concentration of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in FIV-positive cats. Sixteen adults FIV-positive asymptomatic cats were included in the study. WB samples were drawn and the PRP was obtained by centrifugation at 265g for 10 min. Erythrocyte and leukocyte, platelets, and mean platelet volume (MPV) were determined both in WB and in PRP. PDGF-BB and TGF-β1 concentrations were additionally determined in PRP. Platelet concentration increased 1.1 times in PRP fraction compared to WB, but no significant differences were reported. MPV was statistically higher in WB than in PRP (p = 0.001). Erythrocytes and leukocytes counts were decreased by 99% and 92%, respectively in the PRP fraction (p < 0.001). Regarding TGF-ß1, a higher concentration was shown in the PRP (p < 0.02). Although the product obtained could not be classified as PRP according to the PRGF®-Endoret® methodology, based on the drastic reduction of RBC and WBC, the PLT concentrate is of high purity.
URI: http://hdl.handle.net/10637/15896
Rights : http://creativecommons.org/licenses/by-nc/4.0/deed.es
Open Access
ISSN: 0034-5288
1532-2661 (Electrónico)
Supported by: Acuerdo Transformativo – 2024
Issue Date: Mar-2024
Center : Universidad Cardenal Herrera-CEU
Appears in Collections:Dpto. Medicina y Cirugía Animal





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