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Method for triglyceride measurement in small amounts of plasma.

Título : Method for triglyceride measurement in small amounts of plasma.
Autor : Ramírez, J. I.
Herrera Castillón, Emilio.
Llobera i Sande, Miquel
Resumen : A method is described for determining triacylglyceride concentration in small amounts of plasma. After ethanolic-KOH digestion of diluted plasma aliquots, samples were neutralized with MgSO4 and gly~erol was fluorimetrically assayed in supematants by the coupling of glycerokinase, pyruvate kinase, and lactate dehydrogenase catalyzed reactions. Values were corrected by free glycerol present in the non-digested samples. Digestions were performed at different times and temperatures in order to establish optimum conditions for recoveries and reproducibility. Parallel determinations before and after phospholipid removal showed that their presence in plasma did not interfere with the obtained values. This method is especially useful for running many samples in parallel and for determinations in small experimental animals in which the amount of plasma is very limited.
Descripción : En: Revista Española de Fisiología ISSN 0034-9402 1983. N. 39, pp 327-331
URI : http://hdl.handle.net/10637/871
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Fecha de publicación : 19-sep-1983
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





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