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dc.contributor.authorBarbas Arribas, Coral.-
dc.creatorVillaseñor Solis, Alma Cristina-
dc.creatorGarcía Fernández, Antonia-
dc.creatorPosma, Joram M.-
dc.creatorFernández López, Mariano-
dc.creatorNicholas, Andreas J.-
dc.creatorModi, Neena-
dc.creatorHolmes, Elaine-
dc.creatorBarbas Arribas, Coral.-
dc.date2014es
dc.date.accessioned2015-09-16T04:00:13Z-
dc.date.available2015-09-16T04:00:13Z-
dc.date.issued2014-09-16-
dc.identifier000000624695es
dc.identifier.urihttp://hdl.handle.net/10637/7660-
dc.descriptionEn: Analytical Chemistry. ESSN. 1520-6882. v. 86 (16) 2014 : 8245-8252es
dc.description.abstractBreast milk (BM) is a biofluid, which has a fundamental role in early-life nutrition and directly impacts on growth, neurodevelopment and health. Global metabolic profiling is increasingly being utilized to characterize complex metabolic changes in biological samples. However, in order to achieve broad metabolite coverage, it is necessary to employ more than one analytical platform, typically requiring multiple sample preparation protocols. In an effort to improve analytical efficiency and retain comprehensive coverage of the metabolome, a new extraction methodology was developed that successfully retains metabolites from BM in a single-phase using an optimized methyl-tert-butyl ether solvent system. We conducted this single-phase extraction procedure on a representative pool of BM, and characterized the metabolic composition using LC-QTOF-MS and GC-Q-MS for polar and lipidic metabolites. To ensure that the extraction method was reproducible and fit-for-purpose, the analytical procedure was evaluated on both platforms using 18 metabolites selected to cover a range of chromatographic retention times and biochemical classes. Having validated the method, the metabolic signature of BM composition was mapped as a metabolic reaction network highlighting interconnected biological pathways and showing that the LC-MS and GC-MS platforms targeted largely different domains of the network. Subsequently, the same protocol was applied to ascertain compositional differences between BM at week 1 (n=10) and 4 weeks (n=9) post-partum. This single-phase approach is more efficient in terms of time, simplicity, cost and sample volume than the existing two phase methods, and will be suited to high-throughput metabolic profiling studies of BM.en-EN
dc.formatapplication/pdfes
dc.language.isoenes
dc.relationFinanciado con cargo a proyectos de I+D nacional con referencia CTQ 2011-23562es
dc.relationfinancial support from Ministry of Science and Innovation MICINN CTQ2011-23562. IGP thanks a NIHR postgraduate research fellowship.en-EN
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.eses
dc.subjectMetabolitos.es
dc.titleBreast milk metabolome characterization in a single phase extraction, multiplatform analytical approach.en-EN
dc.typeArtículoes
dc.description.versionPost-print del autores
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU-
europeana.isShownAthttp://hdl.handle.net/10637/7660-
europeana.objecthttp://repositorioinstitucional.ceu.es/visor/libros/624695/thumb_europeana/624695.jpg-
europeana.providerHispana-
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/-
europeana.typeTEXT-
dc.centroUniversidad San Pablo-CEU-
Aparece en las colecciones: Facultad de Farmacia




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