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dc.contributor.otherGrupo: Parasitología e Inmunología molecular con aplicación biotecnológica, diagnóstica y terapéutica (PARINM)-
dc.contributor.otherUniversidad San Pablo-CEU. Departamento de Ciencias Farmacéuticas y de la Salud-
dc.creatorSáez Álvarez, Yanira-
dc.creatorArias, Armando-
dc.creatorDel Águila, Carmen-
dc.creatorAgudo Torres, Rubén-
dc.date.accessioned2024-03-21T15:00:26Z-
dc.date.available2024-03-21T15:00:26Z-
dc.date.issued2019-04-01-
dc.identifier.citationSáez-Álvarez, Y., del Águila, C., Agudo, R., & Arias, A. (2019). Development of a fluorescence-based method for the rapid determination of Zika virus polymerase activity and the screening of antiviral drugs. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-41998-1es_ES
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10637/15658-
dc.description.abstractZika virus (ZIKV) is an emerging pathogen that has been associated with large numbers of cases of severe neurologic disease, including Guillain-Barré syndrome and microcephaly. Despite its recent establishment as a serious global public health concern there are no licensed therapeutics to control this virus. Accordingly, there is an urgent need to develop methods for the high-throughput screening of antiviral agents. We describe here a fuorescence-based method to monitor the real-time polymerization activity of Zika virus RNA-dependent RNA polymerase (RdRp). By using homopolymeric RNA template molecules, de novo RNA synthesis can be detected with a fuorescent dye, which permits the specifc quantifcation and kinetics of double-strand RNA formation. ZIKV RdRp activity detected using this fuorescence-based assay positively correlated with traditional assays measuring the incorporation of radiolabeled nucleotides. We also validated this method as a suitable assay for the identifcation of ZIKV inhibitors targeting the viral polymerase using known broad-spectrum inhibitors. The assay was also successfully adapted to detect RNA polymerization activity by diferent RdRps, illustrated here using purifed RdRps from hepatitis C virus and foot-and-mouth disease virus. The potential of fuorescence-based approaches for the enzymatic characterization of viral polymerases, as well as for high-throughput screening of antiviral drugs, are discussed.en_EN
dc.language.isoenen_EN
dc.publisherNature Publishing Groupes_ES
dc.relation.ispartofScientific Reports-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.titleDevelopment of a fluorescence-based method for the rapid determination of Zika virus polymerase activity and the screening of antiviral drugsen_EN
dc.typeArtículo-
dc.identifier.doi10.1038/s41598-019-41998-1-
dc.centroUniversidad San Pablo-CEU-
Aparece en las colecciones: Facultad de Farmacia




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