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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.other | Universidad San Pablo-CEU. Facultad de Farmacia. Departamento de Ciencias Farmacéuticas y de la Salud | - |
dc.creator | Sánchez Jiménez, Patricia | - |
dc.creator | Elizalde-Horcada, Marcos | - |
dc.creator | Sanz García, Ancor | - |
dc.creator | Granero Cremades, Inmaculada | - |
dc.creator | Toledo, María de | - |
dc.creator | Pullido, Paloma | - |
dc.creator | Navas, Marta | - |
dc.creator | Gago-Veiga, Beatriz | - |
dc.creator | Alonso-Guirado, Lola | - |
dc.creator | Alonso Cerezo, María Concepción | - |
dc.creator | Nava Cedeño, Desiréé | - |
dc.creator | Abad Santos, Francisco | - |
dc.creator | Torres Díaz, Cristina Virginia | - |
dc.creator | Ovejero Benito, María del Carmen | - |
dc.date.accessioned | 2024-01-17T18:57:54Z | - |
dc.date.available | 2024-01-17T18:57:54Z | - |
dc.date.issued | 2023-01-05 | - |
dc.identifier.citation | Sánchez-Jiménez, P., Elizalde-Horcada, M., Sanz-García, A. et al. DNA Methylation Description of Hippocampus, Cortex, Amygdala, and Blood of Drug-Resistant Temporal Lobe Epilepsy. Mol Neurobiol 60, 2070–2085 (2023). https://doi.org/10.1007/s12035-022-03180-z | es_ES |
dc.identifier.issn | 1559-1182 | - |
dc.identifier.uri | http://hdl.handle.net/10637/14941 | - |
dc.description.abstract | Epigenetic changes such as DNA methylation were observed in drug-resistant temporal lobe epilepsy (DR-DR-TLE), a disease that affects 25-30% of epilepsy patients. The main objective is to simultaneously describe DNA methylation patterns associated with DR-TLE in hippocampus, amygdala, surrounding cortex to the epileptogenic zone (SCEZ), and peripheral blood. Methods: An Illumina Infinium MethylationEPIC BeadChip array was performed in 19 DR-TLE patients and 10 postmortem non-epileptic controls. Results: Overall, 32, 59, and 3210 differentially methylated probes (DMPs) were associated with DR-TLE in the hippocampus, amygdala, and SCEZ, respectively. These DMPs affected genes were involved in neurotrophic and calcium signaling in the hippocampus, and voltage-gated channels in SCEZ, among others. One of the hippocampus DMPs (cg26834418 (CHORDC1)) showed a strong blood-brain correlation with BECon and IMAGE-CpG, suggesting that it could be a potential surrogate peripheral biomarker of DR-TLE. Moreover, in three of the top SCEZ’s DMPs (SHANK3, SBF1 and MCF2L) methylation status was verified with methylation-specific qPCR. The differentially methylated CpGs were classified in DMRs: 2 in the hippocampus, 12 in the amygdala, and 531 in the SCEZ. Conclusion: We identified genes that had not been associated to DR-TLE so far such as TBX5, EXOC7 and WRHN. The area with more DMPs associated with DR-TLE was the SCEZ, some of them related to voltage-gated channels. The DMPs found in the amygdala were involved in inflammatory processes. We also found a potential surrogate peripheral biomarker of DR-TLE. Thus, these results provide new insights into epigenetic modifications involved in DR-TLE.. | en_EN |
dc.format | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | Springer Link | - |
dc.relation.ispartof | Molecular Neurobiology | - |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | - |
dc.subject | DNA methylation array | en_EN |
dc.subject | Drug-resistant epilepsy | en_EN |
dc.subject | Epigenome-wide association study (EWAS) | en_EN |
dc.subject | Epilepsy biomarkers | en_EN |
dc.subject | Epigenetic | en_EN |
dc.title | DNA methylation description of hippocampus, cortex, amygdala, and blood of Drug Resistant-Temporal Lobe Epilepsy | en_EN |
dc.type | Artículo | - |
dc.identifier.doi | 10.1007/s12035-022-03180-z | - |
dc.date.endEmbargo | 2024-04-01 | - |
dc.centro | Universidad San Pablo-CEU | - |
Aparece en las colecciones: | Facultad de Farmacia |
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