Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/14872

Development and validation of a rapid UV-HPLC method for the determination of anidulafungin in perfusion solution


Vista previa

Ver/Abrir:
 Development_Estan_CPA_2017.pdf
169,53 kB
Adobe PDF
Título : Development and validation of a rapid UV-HPLC method for the determination of anidulafungin in perfusion solution
Autor : Estan Cerezo, Gabriel
Rodríguez Lucena, Francisco José
Murcia López, Ana Cristina
Matoses Chirivella, Carmen
Escudero Ortiz, Vanesa
Andújar Mateos, Arantxa
Navarro Ruiz, Andrés
Materias: Tratamiento médicoMedical treatmentMedicamentoDrugs
Editorial : Bentham Science
Citación : Estan-Cerezo, G., Rodriguez-Lucena, F.J., Murcia-López, A.C., Matoses Chirivella, C., Escudero-Ortiz, V., Andujar-Mateos, A. & Navarro-Ruiz, A. (2017). Development and validation of a rapid UV-HPLC method for the determination of anidulafungin in perfusion solution. Current Pharmaceutical Analysis, vol. 13, i. 3, pp. 314 - 320. DOI: https://doi.org/10.2174/1573412912666160929170016
Resumen : Background: Anidulafungin is an antifungal agent. The development of determination techniques can be a useful tool to realize stability and quality control studies. Methods: The determination was performed on an analytical Mediterranea SEA18 (15x0.4 cm, 5 μm) C18 column at 35 ºC. The selected wavelength was 304 nm. The mobile phase was a mixture of 0.037 M sodium dihydrogen phosphate buffer, acetonitrile and methanol (40:50:10, v/v/v) at a flow rate of 2.0 mL min–1. Dasatinib (12.5 μg mL-1) was used as internal standard. Results: The assay enables the measurement of anidulafungin with a linear calibration curve (r2= 0.999) over the concentration range 15–300 μg mL-1. Accuracy, intra-day repeatability (n = 5), and inter-day precision (n = 3) were found to be satisfactory, being the accuracy 5.8% and precisions were intra-day and inter-day, 0.6% and 4.2%, respectively. Conclusions: A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method with ultra violet detection has been developed for quantification of anidulafungin in perfusion solution. The retention time was clearly minor than the previous published HPLC determination methods of anidulafungin.
Descripción : This is the peer reviewed version of the following article: Estan-Cerezo, G., Rodriguez-Lucena, F.J., Murcia-López, A.C., Matoses Chirivella, C., Escudero-Ortiz, V., Andujar-Mateos, A. & Navarro-Ruiz, A. (2017). Development and validation of a rapid UV-HPLC method for the determination of anidulafungin in perfusion solution. Current Pharmaceutical Analysis, vol. 13, i. 3, pp. 314 - 320. The published manuscript is available at EurekaSelect via https://doi.org/10.2174/1573412912666160929170016
URI : http://hdl.handle.net/10637/14872
ISSN : 1573-4129
1875-676X (Electrónico)
Fecha de publicación : 2017
Centro : Universidad Cardenal Herrera-CEU
Aparece en las colecciones: Dpto. Ciencias Biomédicas





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.