Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/1033

Development and validation of a capillary electrophoresis method for short chain organic acids measurement in natural rubber latex.

Título : Development and validation of a capillary electrophoresis method for short chain organic acids measurement in natural rubber latex.
Autor : Galli, Verónica
Barbas Arribas, Coral.
Olmo Izquierdo, Nuria del
Materias: Validation.Rubber.Latex.Organic acids.Fatty acids.
Resumen : Short-chain organic acid contents in serum of natural latex are interesting to measure and capillary electrophoresis (CE) has proved to be a good tool for their study. In the present work a method has been developed to identify the short-chain organic acids present in sera of natural rubber latex (oxalic, formic, fumaric, aconitic, succinic, malic, glutaric, citric, acetic, glycollic, propionic and quinic acids), the separation was optimised and the quantification method validated. The separation was performed on a CE system with UV detection at 200 nm. The separation was carried out with an uncoated fused-silica capillary (57 cm350 mm I.D.) and was operated at 210 kV potential. The separation buffers were prepared with 0.5 M H PO , 0.5 mM cetyltrimethylammonium bromide and pH adjusted by adding NaOH to 6.25 except for propionic acid 3 4 which was better measured at pH 7.00. Validation parameters are adequate and limits of detection range from 0.005 mM to 1.6 mM. Short-chain organic acids were measured with this method in sera of three different types of latex.
Descripción : En: Journal of chromatography A, ISSN 0021-9673, 2000. n. 894 : 135-144 p.
URI : http://hdl.handle.net/10637/1033
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Fecha de publicación : 19-sep-2000
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.