A rapid and sensitive method for HPLC cholesterol determination in bile.

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dc.centroUniversidad San Pablo-CEU
dc.contributor.authorBocos de Prada, Carlos
dc.contributor.authorCastro, Mario
dc.contributor.authorOrozco, Enrique
dc.contributor.authorContreras, Juan Antonio
dc.contributor.authorHerrera Castillón, Emilio
dc.date1992-
dc.date.accessioned2011-09-19T15:39:41Z
dc.date.available2011-09-19T15:39:41Z
dc.date.issued1992-09-19T15:39:41Z
dc.descriptionEn: Revista Española de Fisiología, ISSN 0034-9402 1992. N. 48, pp 211-224-
dc.description.abstractA relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100 % values. It is highly sensiti\·e and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids.en_EN
dc.description.abstractSe describe un rnétodo sencillo y rápido para determinar !as concentraciones de colesterol en bilis, basado en la formacion de 3-cetocolestcrol, mediante el tratamiento con colesterol oxidasa, y su mcdida por cromatografia de alta resolucion. El metodo evita !as interferencias con la bilirrubina y presenta una alta reproducibilidad. Los ensayos de recuperacion dan valores del I 00 % . El metodo es de alta sensibilidad y apropiado para cuantificar el colesterol en bilis y en otros fluidos biologicos que comengan bilirrubina.es_ES
dc.formatapplication/pdf-
dc.identifier000000401234-
dc.identifier.urihttp://hdl.handle.net/10637/1047
dc.language.isoen-
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectDeterminación de colesteroles_ES
dc.titleA rapid and sensitive method for HPLC cholesterol determination in bile.-
dc.typeArtículo-
dspace.entity.typePublicationes
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU
europeana.isShownAthttp://hdl.handle.net/10637/1047
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europeana.providerHispana
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/
europeana.typeTEXT
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