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Different hydrolytic efficiencies of adipose tissue lipoprotein lipase on very-low-density lipoprotein subfractions separated by heparin-Sepharose chromatography.

Título : Different hydrolytic efficiencies of adipose tissue lipoprotein lipase on very-low-density lipoprotein subfractions separated by heparin-Sepharose chromatography.
Autor : Gómez Coronado, Diego
Sáez, Guillermo
Lasunción, Miguel Ángel.
Herrera Castillón, Emilio.
Materias: VLDL.Heparin-Sepharose.Apolipoproteins.Lipoprotein lipase.Triacylglycerol hydrolysis.Adipose tissue.
Resumen : Human very-low-density lipoproteins (VLDL) were subfractionated by heparin-Sepharose chromatography into an unbound (:\I and three bound (B, C and D) populations at increasing ionic strengths. Subfractions were characterized regarding their chemic;,[ composition and efficiency of triacylglycerol hydrolysis by rat adipose tissue LPL. The triacylglycerol content decreased, whcrca, the cholesterol and protein contents increased from subfractions A and B to subfraction D. VLDL-D showed the highest ap<' E/apo C ratio, though all the subfractions contained appreciable apo E. Appearance of VLDL-A resulted from exceeding the binding capacity of the column, since practically all its particles eluted at positions of bound VLDL under re-chromatograph~ Subfractions B, C and D stimulated LPL activity on emulsified tri[ 14C]oleoylglycerol to a similar extent, indicating that their 3P'' C-11 content was equally effective activating LPL. Incubation of tri[ 14C]oleoylglycerol labeled VLDL subfractions with fat pad pieces in the presence or absence of heparin resulted in greater hydrolysis and fatty acid uptake for VLDL-B and -C than f.•1 VLDL-D, a pattern observed over a wide range of LPL activities in the media. We conclude: (1) any VLDL particle can intcr;t,1 with heparin, which is consistent with the presence of apo E in all the subfractions, and (2) triacylglycerols in apo E-rich VLDI are less efficiently hydrolyzed by LPL than those in apo E-poor particles. We propose that richness in apo E impairs LPL acu"r upon VLDL and decreases the rate of delivery of fatty acids to peripheral tissues.
Descripción : En: Biochimica et biophysica acta, ISSN 0006-3002 1993. Vol. 1167, pp 70-78
URI : http://hdl.handle.net/10637/855
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Fecha de publicación : 19-sep-1993
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





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