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dc.creatorGómez Coronado, Diego-
dc.creatorSáez, Guillermo-
dc.creatorLasunción, Miguel Ángel.-
dc.creatorHerrera Castillón, Emilio.-
dc.date1993-
dc.date.accessioned2011-09-19T15:39:42Z-
dc.date.available2011-09-19T15:39:42Z-
dc.date.issued1993-09-19T15:39:42Z-
dc.identifier000000400786-
dc.identifier.urihttp://hdl.handle.net/10637/855-
dc.descriptionEn: Biochimica et biophysica acta, ISSN 0006-3002 1993. Vol. 1167, pp 70-78-
dc.description.abstractHuman very-low-density lipoproteins (VLDL) were subfractionated by heparin-Sepharose chromatography into an unbound (:\I and three bound (B, C and D) populations at increasing ionic strengths. Subfractions were characterized regarding their chemic;,[ composition and efficiency of triacylglycerol hydrolysis by rat adipose tissue LPL. The triacylglycerol content decreased, whcrca, the cholesterol and protein contents increased from subfractions A and B to subfraction D. VLDL-D showed the highest ap<' E/apo C ratio, though all the subfractions contained appreciable apo E. Appearance of VLDL-A resulted from exceeding the binding capacity of the column, since practically all its particles eluted at positions of bound VLDL under re-chromatograph~ Subfractions B, C and D stimulated LPL activity on emulsified tri[ 14C]oleoylglycerol to a similar extent, indicating that their 3P'' C-11 content was equally effective activating LPL. Incubation of tri[ 14C]oleoylglycerol labeled VLDL subfractions with fat pad pieces in the presence or absence of heparin resulted in greater hydrolysis and fatty acid uptake for VLDL-B and -C than f.•1 VLDL-D, a pattern observed over a wide range of LPL activities in the media. We conclude: (1) any VLDL particle can intcr;t,1 with heparin, which is consistent with the presence of apo E in all the subfractions, and (2) triacylglycerols in apo E-rich VLDI are less efficiently hydrolyzed by LPL than those in apo E-poor particles. We propose that richness in apo E impairs LPL acu"r upon VLDL and decreases the rate of delivery of fatty acids to peripheral tissues.en_EN
dc.formatapplication/pdf-
dc.language.isoen-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.subjectVLDL.en_EN
dc.subjectHeparin-Sepharose.en_EN
dc.subjectApolipoproteins.en_EN
dc.subjectLipoprotein lipase.en_EN
dc.subjectTriacylglycerol hydrolysis.en_EN
dc.subjectAdipose tissue.en_EN
dc.titleDifferent hydrolytic efficiencies of adipose tissue lipoprotein lipase on very-low-density lipoprotein subfractions separated by heparin-Sepharose chromatography.-
dc.typeArtículo-
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU-
europeana.isShownAthttp://hdl.handle.net/10637/855-
europeana.objecthttp://repositorioinstitucional.ceu.es/visor/libros/400786/thumb_europeana/400786.jpg-
europeana.providerHispana-
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/-
europeana.typeTEXT-
dc.centroUniversidad San Pablo-CEU-
Aparece en las colecciones: Facultad de Farmacia




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