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http://hdl.handle.net/10637/15253
MidA is a putative methyltransferase that is required for mitochondrial complex I function
Title: | MidA is a putative methyltransferase that is required for mitochondrial complex I function |
Authors : | Carilla-Latorre, Sergio Gallardo, M. Esther Annesley, Sarah J. Calvo Garrido, Javier Graña, Osvaldo Accari, Sandra L. Smith, Paige K. Valencia, Alfonso Garesse, Rafael Fisher, Paul R. Escalante, Ricardo |
Keywords: | Dictyostelium; Complex I; MidA; PRO1853; C2orf56; LOC55471; DUF185 |
Publisher: | The Company of Biologists |
Citation: | Carilla-Latorre S, Gallardo ME, Annesley SJ, Calvo-Garrido J, Graña O, Accari SL, Smith PK, Valencia A, Garesse R, Fisher PR, Escalante R. MidA is a putative methyltransferase that is required for mitochondrial complex I function. J Cell Sci. 2010 May 15;123(Pt 10):1674-83. doi: 10.1242/jcs.066076. Epub 2010 Apr 20. PMID: 20406883 |
Abstract: | Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185. Using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex I subunit NDUFS2. Consistent with this, Dictyostelium cells lacking MidA showed a specific defect in complex I activity, and knockdown of human MidA in HEK293T cells resulted in reduced levels of assembled complex I. These results indicate a role for MidA in complex I assembly or stability. A structural bioinformatics analysis suggested the presence of a methyltransferase domain; this was further supported by site-directed mutagenesis of specific residues from the putative catalytic site. Interestingly, this complex I deficiency in a Dictyostelium midA− mutant causes a complex phenotypic outcome, which includes phototaxis and thermotaxis defects. We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology |
URI: | http://hdl.handle.net/10637/15253 |
Rights : | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
ISSN: | 1477-9137 |
Issue Date: | 15-May-2010 |
Center : | Universidad San Pablo-CEU |
Appears in Collections: | Medicina |
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