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MidA is a putative methyltransferase that is required for mitochondrial complex I function


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Title: MidA is a putative methyltransferase that is required for mitochondrial complex I function
Authors : Carilla-Latorre, Sergio
Gallardo, M. Esther
Annesley, Sarah J.
Calvo Garrido, Javier
Graña, Osvaldo
Accari, Sandra L.
Smith, Paige K.
Valencia, Alfonso
Garesse, Rafael
Fisher, Paul R.
Escalante, Ricardo
Keywords: DictyosteliumComplex IMidAPRO1853C2orf56LOC55471DUF185
Publisher: The Company of Biologists
Citation: Carilla-Latorre S, Gallardo ME, Annesley SJ, Calvo-Garrido J, Graña O, Accari SL, Smith PK, Valencia A, Garesse R, Fisher PR, Escalante R. MidA is a putative methyltransferase that is required for mitochondrial complex I function. J Cell Sci. 2010 May 15;123(Pt 10):1674-83. doi: 10.1242/jcs.066076. Epub 2010 Apr 20. PMID: 20406883
Abstract: Dictyostelium and human MidA are homologous proteins that belong to a family of proteins of unknown function called DUF185. Using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex I subunit NDUFS2. Consistent with this, Dictyostelium cells lacking MidA showed a specific defect in complex I activity, and knockdown of human MidA in HEK293T cells resulted in reduced levels of assembled complex I. These results indicate a role for MidA in complex I assembly or stability. A structural bioinformatics analysis suggested the presence of a methyltransferase domain; this was further supported by site-directed mutagenesis of specific residues from the putative catalytic site. Interestingly, this complex I deficiency in a Dictyostelium midA− mutant causes a complex phenotypic outcome, which includes phototaxis and thermotaxis defects. We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology
URI: http://hdl.handle.net/10637/15253
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
ISSN: 1477-9137
Issue Date: 15-May-2010
Center : Universidad San Pablo-CEU
Appears in Collections:Medicina





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