Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/14550

Receptor protein tyrosine phosphatase β/ζ regulates loss of neurogenesis in the mouse hippocampus following adolescent acute ethanol exposure

Title: Receptor protein tyrosine phosphatase β/ζ regulates loss of neurogenesis in the mouse hippocampus following adolescent acute ethanol exposure
Authors : Galán Llario, Milagros
Rodríguez Zapata, María
Gramage Caro, Esther
Vicente Rodríguez, Marta
Fontán Baselga, Teresa
Ovejero Benito, María del Carmen
Pérez García, Carmen
Carrasco, Javier
Moreno Herradón, Marco
Sevillano Fernández, Julio
Ramos Álvarez, María del Pilar
Zapico Rodríguez, José María
De Pascual Teresa, Beatriz
Ramos González, Ana
Herradón Gil-Gallardo, Gonzalo
Keywords: AlcoholNeurotoxicityNeuroinflammationPleiotrophin
Publisher: Elsevier
Citation: Milagros Galán-Llario, María Rodríguez-Zapata, Esther Gramage, Marta Vicente-Rodríguez, Teresa Fontán-Baselga, María Carmen Ovejero-Benito, Carmen Pérez-García, Javier Carrasco, Marco Moreno-Herradón, Julio Sevillano, María Pilar Ramos-Álvarez, José María Zapico, Beatriz de Pascual-Teresa, Ana Ramos, Gonzalo Herradón, Receptor protein tyrosine phosphatase β/ζ regulates loss of neurogenesis in the mouse hippocampus following adolescent acute ethanol exposure, NeuroToxicology, Volume 94, 2023, Pages 98-107, ISSN 0161-813X, https://doi.org/10.1016/j.neuro.2022.11.008.
Abstract: Adolescence is a critical period for brain maturation in which this organ is more vulnerable to the damaging effects of ethanol. Administration of ethanol in mice induces a rapid cerebral upregulation of pleiotrophin (PTN), a cytokine that regulates the neuroinflammatory processes induced by different insults and the behavioral effects of ethanol. PTN binds Receptor Protein Tyrosine Phosphatase (RPTP) β/ζ and inhibits its phosphatase activity, suggesting that RPTPβ/ζ may be involved in the regulation of ethanol effects. To test this hypothesis, we have treated adolescent mice with the RPTPβ/ζ inhibitor MY10 (60 mg/kg) before an acute ethanol (6 g/kg) administration. Treatment with MY10 completely prevented the ethanol-induced neurogenic loss in the hippocampus of both male and female mice. In flow cytometry studies, ethanol tended to increase the number of NeuN+/activated Caspase-3+ cells particularly in female mice, but no significant effects were found. Ethanol increased Iba1+ cell area and the total marked area in the hippocampus of female mice, suggesting sex differences in ethanol-induced microgliosis. In addition, ethanol reduced the circulating levels of IL-6 and IL-10 in both sexes, although this reduction was only found significant in males and not affected by MY10 treatment. Interestingly, MY10 alone increased the total marked area and the number of Iba1+ cells only in the female hippocampus, but tended to reduce the circulating levels of TNF-α only in male mice. In summary, the data identify a novel modulatory role of RPTPβ/ζ on ethanol-induced loss of hippocampal neurogenesis, which seems unrelated to glial and inflammatory responses. The data also suggest sex differences in RPTPβ/ζ function that may be relevant to immune responses and ethanol-induced microglial responses.
URI: http://hdl.handle.net/10637/14550
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Issue Date: 26-Jan-2023
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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