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Genetic sexing strains for the population suppression of the mosquito vector "Aedes aegypti"


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Title: Genetic sexing strains for the population suppression of the mosquito vector "Aedes aegypti"
Authors : Koskinioti, Panagiota
Augustinos, Antonios A.
Carvalho, Danilo O.
Haq, Muhammad Misbah-ul
Pillwax, Gulizar
Durán de la Fuente, Lucía
Salvador Herranz, Gustavo
Keywords: Mosquitoes - Genetics - Control.Mosquitoes - Breeding.Population genetics - Mathematical models.Genética de poblaciones - Modelos matemáticos.Culícidos - Genética - Control.Animals as carriers of disease.Animales vectores.Culícidos - Cría y explotación.Dengue.Zika.
Publisher: The Royal Society
Citation: Koskinioti, P., Augustinos, A.A., Carvalho, D.O., Misbah-ul-Haq, M., Pillwax, G., Duran-de-la-Fuente, L., Salvador-Herranz, G., Argilés-Herrero, R. & Bourtzis, K. (2021). Genetic sexing strains for the population suppression of the mosquito vector "Aedes aegypti". Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 376, i. 1818 (15 feb.), art. 20190808. DOI: http://doi.org/10.1098/rstb.2019.0808
Abstract: Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes.
Description: Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://royalsocietypublishing.org/doi/epdf/10.1098/rstb.2019.0808
En este artículo también participan: Rafael Argilés Herrero y Kostas Bourtzis.
Este artículo pertenece al número que lleva por título "Novel control strategies for mosquito-borne diseases".
URI: http://hdl.handle.net/10637/13694
Rights : http://creativecommons.org/licenses/by/4.0/deed.es
ISSN: 0962-8436
1471-2970 (Electrónico)
Issue Date: 15-Feb-2021
Center : Universidad Cardenal Herrera-CEU
Appears in Collections:Dpto. Proyectos, Teoría y Técnica del Diseño y la Arquitectura





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