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A rapid and sensitive method for HPLC cholesterol determination in bile.


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Title: A rapid and sensitive method for HPLC cholesterol determination in bile.
Authors : Bocos de Prada, Carlos
Castro, Mario
Orozco, Enrique
Contreras, Juan Antonio
Herrera Castillón, Emilio.
Keywords: Determinación de colesterol
Abstract: A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100 % values. It is highly sensiti\·e and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids.

Se describe un rnétodo sencillo y rápido para determinar !as concentraciones de colesterol en bilis, basado en la formacion de 3-cetocolestcrol, mediante el tratamiento con colesterol oxidasa, y su mcdida por cromatografia de alta resolucion. El metodo evita !as interferencias con la bilirrubina y presenta una alta reproducibilidad. Los ensayos de recuperacion dan valores del I 00 % . El metodo es de alta sensibilidad y apropiado para cuantificar el colesterol en bilis y en otros fluidos biologicos que comengan bilirrubina.
Description: En: Revista Española de Fisiología, ISSN 0034-9402 1992. N. 48, pp 211-224
URI: http://hdl.handle.net/10637/1047
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Issue Date: 19-Sep-1992
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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