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dc.creatorRodero, M.es
dc.creatorChivato Pérez, Tomás-
dc.creatorMuro, A.-
dc.creatorCuéllar, Carmen-
dc.date2005es
dc.date.accessioned2013-11-09T05:00:32Z-
dc.date.available2013-11-09T05:00:32Z-
dc.date.issued2005-11-09-
dc.identifier000000581199es
dc.identifier.urihttp://hdl.handle.net/10637/5577-
dc.descriptionEn: Memorias do Instituto Oswaldo Cruz. ISSN 0074-0276. vol. 100, n. 3, 2005, págs 293-301.es
dc.description.abstractAn evaluation of the sensitivity and the specificity of the Anisakis simplex antigens purified by affinity chromatography was performed using sera from patients diagnosed with Anisakis sensitisation and sera from patients previously diagnosed with different helminthic infections. Only the sera of the patients diagnosed with Schistosoma mansoni or Onchocerca volvulus parasitic infections were negative against the A. simplex antigen and its purified fractions (PAK antigen: A. simplex antigen purified using columns prepared with anti-A. simplex rabbit IgG and PAS antigen: PAK antigen purified using columns prepared with anti-Ascaris suum rabbit IgG). However all the sera were positive against the A. suum antigen. In all the sera from the patients diagnosed with Anisakis sensitisation, the antibody levels detected using the purified antigens (PAK and PAS antigens) were lower than the observed using the A. simplex crude extract with the highest diminution in the case of the IgG. When these same sera were tested against the A. simplex crude extract by Western blot, several bands of high molecular masses were observed as well as, intense bands at 60 and/or 40 kDa. A concentration of these last proteins was observed in the PAK and the PAS antigens. When the sensitivity and the specificity determinations were performed, only seven of the 38 patients diagnosed of Anisakis sensitisation were positive, as well as, the sera from the patients diagnosed with parasitisms by Echinococcus granulosus or Fasciola hepatica.en_EN
dc.formatapplication/pdfes
dc.language.isoenes
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.eses
dc.subjectHelmintiasis.es
dc.subjectAnisakiosis.es
dc.subjectAnisakis simplexen_EN
dc.subjectWestern bloten_EN
dc.titleEnzyme-linked immunosorbent assay and Western blot antibody determination in sera from patients diagnosed with different helminthic infections with Anisakis simplex antigen purified by affinity chromatographyes
dc.typeArtículoes
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU-
europeana.isShownAthttp://hdl.handle.net/10637/5577-
europeana.objecthttp://repositorioinstitucional.ceu.es/visor/libros/581199/thumb_europeana/581199.jpg-
europeana.providerHispana-
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/-
europeana.typeTEXT-
Aparece en las colecciones: Medicina




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