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dc.creatorAnado, A.M.es
dc.creatorRodríguez, E.-
dc.creatorGárate, M.T.-
dc.creatorCuéllar, Carmen-
dc.creatorRomarís, F.-
dc.creatorChivato Pérez, Tomás-
dc.creatorRodero, M.-
dc.creatorGonzález Díaz, Humberto-
dc.creatorUbeira, F.M.-
dc.date2010es
dc.date.accessioned2013-11-07T05:01:06Z-
dc.date.available2013-11-07T05:01:06Z-
dc.date.issued2010-11-07-
dc.identifier000000581117es
dc.identifier.urihttp://hdl.handle.net/10637/5558-
dc.descriptionEn: Clinical and vaccine immunology. ISSN 1556-6811. vol. 17, n. 4, 2010, págs 496-502.es
dc.description.abstractCommercially available serological methods for serodiagnosis of human anisakiasis either are poorly specific or do not include some of the most relevant Anisakis allergens. The use of selected recombinant allergens may improve serodiagnosis. To compare the diagnostic and clinical values of enzyme-linked immunosorbent assay (ELISA) methods based on Ani s 1 and Ani s 7 recombinant allergens and of the UniCAP 100 fluorescence enzyme immunoassay (CAP FEIA) system, we tested sera from 495 allergic and 25 non-foodrelated allergic patients. The decay in specific IgE antibodies in serum was also investigated in 15 positive patients over a period of 6 to 38 months. Considering sera that tested positive by either Ani s 1 or Ani s 7 ELISA, the CAP FEIA classified 25% of sera as falsely positive, mainly in the group of patients with the lowest levels of anti-Anisakis IgE antibodies, and 1.28% of positive sera as falsely negative. Considering allergens individually, the overall sensitivities of Ani s 7 ELISA and Ani s 1 ELISA were 94% and 61%, respectively. The results also showed that anti-Anisakis IgE antibodies can be detected in serum for longer with Ani s 1 ELISA than with Ani s 7 ELISA and CAP FEIA (P < 0.01). Our findings suggest that ELISA methods with Ani s 7 and Ani s 1 allergens as targets of IgE antibodies are currently the best option for serodiagnosis of human anisakiasis, combining specificity and sensitivity. The different persistence of anti-Ani s 1 and anti-Ani s 7 antibodies in serum may help clinicians to distinguish between recent and old Anisakis infections.en_EN
dc.formatapplication/pdfes
dc.language.isoenes
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.eses
dc.subjectAnisakiasis.en_EN
dc.subjectEnfermedades parasitarias.es_ES
dc.titleDiagnosing human anisakiasis: recombinant Ani s 1 and Ani s 7 allergens versus the UniCAP 100 fluorescence enzyme immunoassayes
dc.typeArtículoes
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU-
europeana.isShownAthttp://hdl.handle.net/10637/5558-
europeana.objecthttp://repositorioinstitucional.ceu.es/visor/libros/581117/thumb_europeana/581117.jpg-
europeana.providerHispana-
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/-
europeana.typeTEXT-
dc.centroUniversidad San Pablo-CEU-
Aparece en las colecciones: Medicina




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