Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/12999
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.contributor.otherUCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos-
dc.contributor.otherProducción Científica UCH 2018-
dc.creatorQuereda Torres, Juan José-
dc.creatorAndersson, Christopher-
dc.creatorCossart, Pascale-
dc.creatorJohansson, Jörgen-
dc.creatorPizarro Cerdá, Javier-
dc.date2018-
dc.date.accessioned2021-09-14T04:00:24Z-
dc.date.available2021-09-14T04:00:24Z-
dc.date.issued2018-02-06-
dc.identifier.citationQuereda, J.J., Andersson, C., Cossart, P., Johansson, J. & Pizarro‑Cerdá, J. (2018). Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic "Listeria monocytogenes" using the chicken embryo infection model. Veterinary Research, vol. 49, art. 13 (06 feb.). DOI: https://doi.org/10.1186/s13567-017-0496-4-
dc.identifier.issn1297-9716 (Electrónico).-
dc.identifier.urihttp://hdl.handle.net/10637/12999-
dc.descriptionEste artículo se encuentra disponible en la siguiente URL: https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0496-4-
dc.description.abstractMost human listeriosis outbreaks are caused by Listeria monocytogenes evolutionary lineage I strains which possess four exotoxins: a phosphatidylinositol-specific phospholipase C (PlcA), a broad-range phospholipase C (PlcB), listeriolysin O (LLO) and listeriolysin S (LLS). The simultaneous contribution of these molecules to virulence has never been explored. Here, the importance of these four exotoxins of an epidemic lineage I L. monocytogenes strain (F2365) in virulence was assessed in chicken embryos infected in the allantoic cavity. We show that LLS does not play a role in virulence while LLO is required to infect and kill chicken embryos both in wild type transcriptional regulator of virulence PrfA ( PrfAWT) and constitutively active PrfA (PrfA*) backgrounds. We demonstrate that PlcA, a toxin previously considered as a minor virulence factor, played a major role in virulence in a PrfA* background. Interestingly, GFP transcriptional fusions show that the plcA promoter is less active than the hly promoter in vitro, explaining why the contribution of PlcA to virulence could be observed more importantly in a PrfA* background. Together, our results suggest that PlcA might play a more important role in the infectious lifecycle of L. monocytogenes than previously thought, explaining why all the strains of L. monocytogenes have conserved an intact copy of plcA in their genomes.-
dc.formatapplication/pdf-
dc.language.isoes-
dc.language.isoen-
dc.publisherBioMed Central.-
dc.relation.ispartofVeterinary Research, vol. 49, art. 13.-
dc.rightshttp://creativecommons.org/licenses/by/4.0/deed.es-
dc.subjectChickens - Embryology.-
dc.subjectIntoxicación por alimentos.-
dc.subjectFood poisoning.-
dc.subjectPathogenic bacteria.-
dc.subjectListeriosis.-
dc.subjectBacterias patógenas.-
dc.subjectPollos - Embriología.-
dc.titleRole in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic "Listeria monocytogenes" using the chicken embryo infection model-
dc.typeArtículo-
dc.identifier.doihttps://doi.org/10.1186/s13567-017-0496-4-
dc.centroUniversidad Cardenal Herrera-CEU-
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos




Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.