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Título : Nucleotides and AHCC enhance Th1 responses in vitro in "Leishmania"-stimulated-infected murine cells
Autor : Dea Ayuela, María Auxiliadora.
Segarra, Sergi.
Serrano López, Dolores Remedios.
Bolás Fernández, Francisco.
Materias: Leishmaniasis - Tratamiento.Leishmaniasis - Treatment.Enfermedades infecciosas - Tratamiento.Cytokines.Nucleótidos.Nucleotides.Citoquina.Communicable diseases - Treatment.
Fecha de publicación : 27-ago-2020
Editorial : MDPI.
Citación : Dea-Ayuela, M.A., Segarra, S., Serrano, D.R. & Bolás-Fernández, F. (2020). Nucleotides and AHCC enhance Th1 responses in vitro in leishmania-stimulated/infected murine cells. Molecules, vol. 25, i. 17 (27 aug.), art. 3918. DOI: https://doi.org/10.3390/molecules25173918
Resumen : A stronger Th1 (cellular) immune response in canine leishmaniosis (CanL) leads to a better prognosis. Dietary nucleotides plus AHCC® have shown beneficial e ects in dogs with clinical leishmaniosis and in clinically healthy Leishmania-infected dogs. The potential leishmanicidal activity of nucleotides and AHCC was assessed by quantifying nitric oxide (NO) production and replication of parasites. Their e ects on lymphocyte proliferation were studied with and without soluble Leishmania infantum antigen (SLA) stimulation. Cytokine level variations were assessed using naïve and L. infantum-infected macrophages/lymphocytes cocultures. Promastigotes and amastigotes proliferation and NO macrophage production were not directly a ected. Lymphocyte proliferation was significantly enhanced by nucleotides, AHCC, and their combinations only after SLA stimulation. Nucleotides and AHCC significantly increased the production of IL-1 , IL-2, IL-5, IL-9, IL-10, and IL-12 by naïve immune cells. In naïve and L. infantum-infected macrophage/lymphocyte cocultures, nucleotides with or without AHCC led to significant increases in IFN- and TNF- . Given that these cytokines are involved in the e ective Th1 immune response against Leishmania parasites, these mechanisms of action could explain the previously reported in vivo clinical e cacy of such combination and further support the use of nucleotides with or without AHCC in the management of CanL patients.
Descripción : Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1420-3049/25/17/3918
Este artículo pertenece al número especial "Advances of medicinal chemistry against Kinetoplastid Protozoa (Trypanosoma brucei, Trypanosoma cruzi and Leishmania spp.) infections: drug design, synthesis and pharmacology".
URI : http://hdl.handle.net/10637/12672
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 1420-3049 (Electrónico).
Aparece en las colecciones: Dpto. Farmacia

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