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Título : A simplified Iohexol-based method to measure renal function in sheep models of renal disease
Autor : Luis Lima, Sergio.
Más Sanmartín, Carolina.
Rodríguez Rodríguez, Ana Elena.
Porrini, Esteban.
Ortiz Arduan, Alberto.
Gaspari, Flavio.
Martínez Ros, Paula.
González de Bulnes López, Antonio.
Materias: Kidney function tests.Pruebas de función renal.Ovejas - Aparato urinario.Iohexol - Physiological effect.Sheep - Urinary organs.Iohexol - Efectos fisiológicos.
Fecha de publicación : 31-ago-2020
Editorial : MDPI
Citación : Luis-Lima, S., Mas-Sanmartin, C., Rodríguez-Rodríguez, A.E., Porrini, E., Ortiz, A., Gaspari, F. et al. (2020). A simplified Iohexol-based method to measure renal function in sheep models of renal disease. Biology, vol. 9, i. 9 (31 aug.), art. 259. DOI: https://doi.org/10.3390/biology9090259
Resumen : Sheep are highly adequate models for human renal diseases because of their many similarities in the histology and physiology of kidney and pathogenesis of kidney diseases. However, the lack of a simple method to measure glomerular filtration rate (GFR) limits its use as a model of renal diseases. Hence, we aimed to develop a simple method to measure GFR based on the plasma clearance of iohexol by assessing di erent pharmacokinetic models: (a) CL2: two-compartment (samples from 15 to 420 min; reference method); (b) CL1: one-compartment (samples from 60 to 420 min); (c) CLlf : CL1 adjusted by a correction formula and (d) SM: simplified CL2 (15 to 300 min). Specific statistics of agreement were used to test the models against CL2. The agreement between CL1 and CL2 was low, but both CL1f and SM showed excellent agreement with CL2, as indicated by a total deviation index of ~5–6%, a concordance correlation of 0.98–0.99% and a coverage probability of 99–100%, respectively. Hence, the SM approach is preferable due to a reduced number of samples and shorter duration of the procedure; two points that improve animal management and welfare.
Descripción : Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/2079-7737/9/9/259
Este artículo pertenece al número especial "In view of 3R: experimental disease models for studying cardiorenovascular pathology".
En este artículo también participan: Laura Díaz-Martín, Anders Åsberg, Trond Jenssen y Alejandro Jiménez-Sosa.
URI : http://hdl.handle.net/10637/12576
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 2079-7737 (Electrónico).
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos

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