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Título : Reassessing the role of internalin B in Listeria monocytogenes virulence using the epidemic strain F2365
Autor : Quereda Torres, Juan José.
Rodríguez Gómez, Irene Magdalena.
Meza Torres, Jazmín.
Gómez Laguna, Jaime.
Nahori, Marie Anne.
Dussurget, Olivier.
Carrasco Otero, Librado.
Cossart, Pascale.
Pizarro Cerdá, Javier.
Materias: Intoxicación por alimentos.Food poisoning.Proteins.Medical microbiology.Microbiología médica.Listeriosis.Internalin B.Proteinas.
Fecha de publicación : 13-feb-2019
Editorial : Elsevier
Citación : Quereda, JJ., Rodríguez-Gómez, IM., Meza-Torres, J., Gómez-Laguna, J., Nahori, MA., Dussurget, O. et al. (2019). Reassessing the role of internalin B in Listeria monocytogenes virulence using the epidemic strain F2365. Clinical Microbiology and Infection, vol. 25, n. 2 (feb.), pp. 252.e1-252.e4. DOI: https://doi.org/10.1016/j.cmi.2018.08.022
Resumen : Objectives: To investigate the contribution to virulence of the surface protein internalin B (InlB) in the Listeria monocytogenes lineage I strain F2365, which caused a deadly listeriosis outbreak in California in 1985. Methods: The F2365 strain displays a point mutation that hampers expression of InlB. We rescued the expression of InlB in the L. monocytogenes lineage I strain F2365 by introducing a point mutation in the codon 34 (TAA to CAA). We investigated its importance for bacterial virulence using in vitro cell infection systems and a murine intravenous infection model. Results: In HeLa and JEG-3 cells, the F2365 InlBþ strain expressing InlB was z9-fold and z1.5-fold more invasive than F2365, respectively. In livers and spleens of infected mice at 72 hours after infection, bacterial counts for F2365 InlBþ were significantly higher compared to the F2365 strain (z1 log more), and histopathologic assessment showed that the F2365 strain displayed a reduced number of necrotic foci compared to the F2365 InlBþ strain (Mann-Whitney test). Conclusions: InlB plays a critical role during infection of nonpregnant animals by a L. monocytogenes strain from lineage I. A spontaneous mutation in InlB could have prevented more severe human morbidity and mortality during the 1985 California listeriosis outbreak.
Descripción : Este artículo se ha publicado de forma definitiva en: https://www.sciencedirect.com/science/article/pii/S1198743X18306001?via%3Dihub
URI : http://hdl.handle.net/10637/10804
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 1198-743X.
1469-0691 (Electrónico).
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos

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