Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/10760
Título : Changes of salivary biomarkers under different storage conditions : peffects of temperature and length of storage
Autor : Barranco Benacloch, Tomás.
Peres Rubio, Camila.
Tvarijonaviciute, Asta.
Rubio Zaragoza, Mónica.
Damiá Giménez, Elena.
Lamy, Elsa.
Cugat Bertomeu, Ramón.
Cerón Madrigal, José Joaquín.
Tecles, Fernando.
Escribano Tortosa, Damián.
Materias: Estrés oxidativo.Oxidative stress.Marcadores bioquímicos.Saliva - Análisis.Biochemical markers.Saliva - Analysis.Enzimas - Análisis.Enzymes - Analysis.
Fecha de publicación : 15-feb-2019
Editorial : Croatian Society of Medical Biochemistry.
Citación : Barranco, T., Rubio, CP., Tvarijonaviciute, A., Rubio, M., Damia, E., Lamy, E. et al. (2019). Changes of salivary biomarkers under different storage conditions : peffects of temperature and length of storage. Biochemia Medica, vol. 29, n. 1 (15 feb.), art. 010706. DOI: https://doi.org/10.11613/BM.2019.010706
Resumen : Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.
Descripción : Este artículo se ha publicado de forma definitiva en: https://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.2019.010706
En este artículo también participan: Camila P Rubio, Asta Tvarijonaviciute, Mónica Rubio, Elena Damia, Elsa Lamy, Ramón Cugat, José J. Cerón, Fernando Tecles y Damián Escribano.
URI : http://hdl.handle.net/10637/10760
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 1330-0962
1846-7482 (Electrónico)
Aparece en las colecciones: Dpto. Medicina y Cirugía Animal

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Changes_Barranco_BM_2019.pdf139,24 kBAdobe PDFVisualizar/Abrir



Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.