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Título : Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party / Elisa Maiques ... [et al.].
Autor : Maiques Fernández, Elisa.
Quiles Puchalt, Nuria.
Donderis, Jorge.
Ciges Tomás, J. Rafael.
Alite, Christian.
Bowring, Janine.
Humphrey, Suzanne.
Penadés Casanova, José Rafael.
Marina, Alberto.
Materias: Estafilococos.Staphylococcus.Bacteriología médica.Pathogenic bacteria.Medical bacteriology.Medical microbiology.Bacterias patógenas.Microbiología médica.
Fecha de publicación : 2-jun-2016
Editorial : Oxford University Press.
Citación : Maiques, E., Quiles-Puchalt, N., Donderis, J., Ciges-Tomas, JR., Alite, C., Bowring, JZ. et al. (2016). Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party. Nucleic Acids Research, vol. 44, n. 11 (jun. 2016), pp. 5457-5469. DOI: https://doi.org/10.1093/nar/gkw317
Resumen : We have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.
Descripción : Este artículo se encuentra disponible en la siguiente URL: https://academic.oup.com/nar/article/44/11/5457/2468309
URI : http://hdl.handle.net/10637/10627
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 0305-1048
1362-4962 (Electrónico)
Aparece en las colecciones: Dpto. Ciencias Biomédicas




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