Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision

dc.centroUniversidad Cardenal Herrera-CEU
dc.contributor.authorZamora Caballero, Sara
dc.contributor.authorQuiles Puchalt, Nuria
dc.contributor.authorChmielowska, Cora
dc.contributor.authorBrady, Aisling
dc.contributor.authorGallego del Sol, Francisca
dc.contributor.authorMancheño Bonillo, Javier
dc.contributor.authorFelipe Ruiz, Alonso
dc.contributor.authorMeijer, Wilfried J. J.
dc.contributor.authorMarina, Alberto
dc.contributor.authorPenadés Casanova, José Rafael
dc.contributor.otherUCH. Departamento de Ciencias Biomédicas
dc.date.accessioned2024-01-31T11:55:28Z
dc.date.available2024-01-31T11:55:28Z
dc.date.issued2024-01-04
dc.description.abstractPhages can use a small-molecule communication arbitrium system to coordinate lysis–lysogeny decisions, but the underlying mechanism remains unknown. Here we determined that the arbitrium system in Bacillus subtilis phage phi3T modulates the bacterial toxin–antitoxin system MazE–MazF to regulate the phage life cycle. We show that phi3T expresses AimX and YosL, which bind to and inactivate MazF. AimX also inhibits the function of phi3T_93, a protein that promotes lysogeny by binding to MazE and releasing MazF. Overall, these mutually exclusive interactions promote the lytic cycle of the phage. After several rounds of infection, the phage-encoded AimP peptide accumulates intracellularly and inactivates the phage antiterminator AimR, a process that eliminates aimX expression from the aimP promoter. Therefore, when AimP increases, MazF activity promotes reversion back to lysogeny, since AimX is absent. Altogether, our study reveals the evolutionary strategy used by arbitrium to control lysis–lysogeny by domesticating and fine-tuning a phage-defence mechanism.es_ES
dc.identifier.citationZamora-Caballero, S., Chmielowska, C., Quiles-Puchalt, N., Brady, A., Del Sol, F.G., Mancheño-Bonillo, J., Felipe-Ruíz, A., Meijer, W. J.J., Penadés, J.R. & Marina, A. (2024). Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision. Nature Microbiology, vol. 9, i. 1 (jan.), pp. 161–172. DOI: https://doi.org/10.1038/s41564-023-01550-4es_ES
dc.identifier.doihttps://doi.org/10.1038/s41564-023-01550-4
dc.identifier.issn2058-5276 (Electrónico)
dc.identifier.urihttp://hdl.handle.net/10637/15244
dc.language.isoenes_ES
dc.publisherSpringer Naturees_ES
dc.relationEste artículo de investigación ha sido financiado por Ministerio de Ciencia e Innovación del Gobierno de España (PID2019-108541GB-I00 y PID2022-137201NB-I00), por la Generalitat Valenciana (PROMETEO/2020/012) y por la European Commission NextGenerationEU fund (EU 2020/2094) a través de la CSIC’s Global Health Platform (PTI Salud Global). También ha recibido ayudas del Medical Research Council (UK) (MR/M003876/1, MR/V000772/1 y MR/S00940X/1), del Biotechnology and Biological Sciences Research Council (BBSRC, UK) (BB/N002873/1, BB/V002376/1 y BB/S003835/1), del ERC-ADG-2014 Proposal no. 670932 Dut-signal (de la EU) y del Wellcome Trust (201531/Z/16/Z). Finalmente, se recibió una beca predoctoral del Ministerio de Universidades del Gobierno de España (FPU19/00433).
dc.relation.ispartofNature Microbiology, vol. 9, i. 1 (jan.)
dc.relation.projectIDPID2019-108541GB-I00
dc.relation.projectIDPID2022-137201NB-I00
dc.relation.projectIDPROMETEO/2020/012
dc.relation.projectIDEU 2020/2094
dc.relation.projectIDFPU19/00433
dc.relation.projectID201531/Z/16/Z
dc.relation.projectIDMR/M003876/1
dc.relation.projectIDMR/V000772/1
dc.relation.projectIDMR/S00940X/1
dc.relation.projectIDBB/N002873/1
dc.relation.projectIDBB/V002376/1
dc.relation.projectIDBB/S003835/1
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectCélulaes_ES
dc.subjectCellses_ES
dc.subjectBiología celulares_ES
dc.subjectCell biologyes_ES
dc.subjectBiofísicaes_ES
dc.subjectBiophysicses_ES
dc.subjectEcología microbianaes_ES
dc.subjectMicrobial ecologyes_ES
dc.subjectBiología moleculares_ES
dc.subjectMolecular biologyes_ES
dc.titleAntagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decisiones_ES
dc.typeArtículoes_ES
dspace.entity.typePublicationes
relation.isAuthorOfPublication0c0506dc-1088-431a-97bb-13c7cc6cbe48
relation.isAuthorOfPublication34f94146-596c-44e2-8014-58df1c280bea
relation.isAuthorOfPublication.latestForDiscovery0c0506dc-1088-431a-97bb-13c7cc6cbe48

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