Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision
dc.centro | Universidad Cardenal Herrera-CEU | |
dc.contributor.author | Zamora Caballero, Sara | |
dc.contributor.author | Quiles Puchalt, Nuria | |
dc.contributor.author | Chmielowska, Cora | |
dc.contributor.author | Brady, Aisling | |
dc.contributor.author | Gallego del Sol, Francisca | |
dc.contributor.author | Mancheño Bonillo, Javier | |
dc.contributor.author | Felipe Ruiz, Alonso | |
dc.contributor.author | Meijer, Wilfried J. J. | |
dc.contributor.author | Marina, Alberto | |
dc.contributor.author | Penadés Casanova, José Rafael | |
dc.contributor.other | UCH. Departamento de Ciencias Biomédicas | |
dc.date.accessioned | 2024-01-31T11:55:28Z | |
dc.date.available | 2024-01-31T11:55:28Z | |
dc.date.issued | 2024-01-04 | |
dc.description.abstract | Phages can use a small-molecule communication arbitrium system to coordinate lysis–lysogeny decisions, but the underlying mechanism remains unknown. Here we determined that the arbitrium system in Bacillus subtilis phage phi3T modulates the bacterial toxin–antitoxin system MazE–MazF to regulate the phage life cycle. We show that phi3T expresses AimX and YosL, which bind to and inactivate MazF. AimX also inhibits the function of phi3T_93, a protein that promotes lysogeny by binding to MazE and releasing MazF. Overall, these mutually exclusive interactions promote the lytic cycle of the phage. After several rounds of infection, the phage-encoded AimP peptide accumulates intracellularly and inactivates the phage antiterminator AimR, a process that eliminates aimX expression from the aimP promoter. Therefore, when AimP increases, MazF activity promotes reversion back to lysogeny, since AimX is absent. Altogether, our study reveals the evolutionary strategy used by arbitrium to control lysis–lysogeny by domesticating and fine-tuning a phage-defence mechanism. | es_ES |
dc.identifier.citation | Zamora-Caballero, S., Chmielowska, C., Quiles-Puchalt, N., Brady, A., Del Sol, F.G., Mancheño-Bonillo, J., Felipe-Ruíz, A., Meijer, W. J.J., Penadés, J.R. & Marina, A. (2024). Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision. Nature Microbiology, vol. 9, i. 1 (jan.), pp. 161–172. DOI: https://doi.org/10.1038/s41564-023-01550-4 | es_ES |
dc.identifier.doi | https://doi.org/10.1038/s41564-023-01550-4 | |
dc.identifier.issn | 2058-5276 (Electrónico) | |
dc.identifier.uri | http://hdl.handle.net/10637/15244 | |
dc.language.iso | en | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.relation | Este artículo de investigación ha sido financiado por Ministerio de Ciencia e Innovación del Gobierno de España (PID2019-108541GB-I00 y PID2022-137201NB-I00), por la Generalitat Valenciana (PROMETEO/2020/012) y por la European Commission NextGenerationEU fund (EU 2020/2094) a través de la CSIC’s Global Health Platform (PTI Salud Global). También ha recibido ayudas del Medical Research Council (UK) (MR/M003876/1, MR/V000772/1 y MR/S00940X/1), del Biotechnology and Biological Sciences Research Council (BBSRC, UK) (BB/N002873/1, BB/V002376/1 y BB/S003835/1), del ERC-ADG-2014 Proposal no. 670932 Dut-signal (de la EU) y del Wellcome Trust (201531/Z/16/Z). Finalmente, se recibió una beca predoctoral del Ministerio de Universidades del Gobierno de España (FPU19/00433). | |
dc.relation.ispartof | Nature Microbiology, vol. 9, i. 1 (jan.) | |
dc.relation.projectID | PID2019-108541GB-I00 | |
dc.relation.projectID | PID2022-137201NB-I00 | |
dc.relation.projectID | PROMETEO/2020/012 | |
dc.relation.projectID | EU 2020/2094 | |
dc.relation.projectID | FPU19/00433 | |
dc.relation.projectID | 201531/Z/16/Z | |
dc.relation.projectID | MR/M003876/1 | |
dc.relation.projectID | MR/V000772/1 | |
dc.relation.projectID | MR/S00940X/1 | |
dc.relation.projectID | BB/N002873/1 | |
dc.relation.projectID | BB/V002376/1 | |
dc.relation.projectID | BB/S003835/1 | |
dc.rights | open access | |
dc.rights.cc | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | Célula | es_ES |
dc.subject | Cells | es_ES |
dc.subject | Biología celular | es_ES |
dc.subject | Cell biology | es_ES |
dc.subject | Biofísica | es_ES |
dc.subject | Biophysics | es_ES |
dc.subject | Ecología microbiana | es_ES |
dc.subject | Microbial ecology | es_ES |
dc.subject | Biología molecular | es_ES |
dc.subject | Molecular biology | es_ES |
dc.title | Antagonistic interactions between phage and host factors control arbitrium lysis–lysogeny decision | es_ES |
dc.type | Artículo | es_ES |
dspace.entity.type | Publication | es |
relation.isAuthorOfPublication | 0c0506dc-1088-431a-97bb-13c7cc6cbe48 | |
relation.isAuthorOfPublication | 34f94146-596c-44e2-8014-58df1c280bea | |
relation.isAuthorOfPublication.latestForDiscovery | 0c0506dc-1088-431a-97bb-13c7cc6cbe48 |
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