Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic "Listeria monocytogenes" using the chicken embryo infection model

dc.centroUniversidad Cardenal Herrera-CEU
dc.contributor.authorQuereda Torres, Juan José
dc.contributor.authorAndersson, Christopher
dc.contributor.authorCossart, Pascale
dc.contributor.authorJohansson, Jörgen
dc.contributor.authorPizarro Cerdá, Javier
dc.contributor.otherUCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos
dc.contributor.otherProducción Científica UCH 2018
dc.date2018
dc.date.accessioned2021-09-14T04:00:24Z
dc.date.available2021-09-14T04:00:24Z
dc.date.issued2018-02-06
dc.descriptionEste artículo se encuentra disponible en la siguiente URL: https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0496-4
dc.description.abstractMost human listeriosis outbreaks are caused by Listeria monocytogenes evolutionary lineage I strains which possess four exotoxins: a phosphatidylinositol-specific phospholipase C (PlcA), a broad-range phospholipase C (PlcB), listeriolysin O (LLO) and listeriolysin S (LLS). The simultaneous contribution of these molecules to virulence has never been explored. Here, the importance of these four exotoxins of an epidemic lineage I L. monocytogenes strain (F2365) in virulence was assessed in chicken embryos infected in the allantoic cavity. We show that LLS does not play a role in virulence while LLO is required to infect and kill chicken embryos both in wild type transcriptional regulator of virulence PrfA ( PrfAWT) and constitutively active PrfA (PrfA*) backgrounds. We demonstrate that PlcA, a toxin previously considered as a minor virulence factor, played a major role in virulence in a PrfA* background. Interestingly, GFP transcriptional fusions show that the plcA promoter is less active than the hly promoter in vitro, explaining why the contribution of PlcA to virulence could be observed more importantly in a PrfA* background. Together, our results suggest that PlcA might play a more important role in the infectious lifecycle of L. monocytogenes than previously thought, explaining why all the strains of L. monocytogenes have conserved an intact copy of plcA in their genomes.
dc.formatapplication/pdf
dc.identifier.citationQuereda, J.J., Andersson, C., Cossart, P., Johansson, J. & Pizarro‑Cerdá, J. (2018). Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic "Listeria monocytogenes" using the chicken embryo infection model. Veterinary Research, vol. 49, art. 13 (06 feb.). DOI: https://doi.org/10.1186/s13567-017-0496-4
dc.identifier.doihttps://doi.org/10.1186/s13567-017-0496-4
dc.identifier.issn1297-9716 (Electrónico).
dc.identifier.urihttp://hdl.handle.net/10637/12999
dc.language.isoes
dc.language.isoen
dc.publisherBioMed Central.
dc.relation.ispartofVeterinary Research, vol. 49, art. 13.
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectChickens - Embryology.
dc.subjectIntoxicación por alimentos.
dc.subjectFood poisoning.
dc.subjectPathogenic bacteria.
dc.subjectListeriosis.
dc.subjectBacterias patógenas.
dc.subjectPollos - Embriología.
dc.titleRole in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic "Listeria monocytogenes" using the chicken embryo infection model
dc.typeArtículo
dspace.entity.typePublicationes
relation.isAuthorOfPublication86645d6c-6972-471f-a8ab-8a39056743ad
relation.isAuthorOfPublication.latestForDiscovery86645d6c-6972-471f-a8ab-8a39056743ad

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