Detection of equine herpesvirus-1 (EHV-1) in urine samplesduring outbreaks of equine herpesvirus myeloencephalopathy

Simple item page
dc.centroUniversidad Cardenal Herrera-CEU
dc.contributor.authorVelloso Álvarez, Ana
dc.contributor.authorJosé Cunilleras, E.
dc.contributor.authorCuesta Torrado, María de la
dc.contributor.authorDorrego Rodríguez, Abel
dc.contributor.authorSantiago Llorente, Isabel
dc.contributor.authorTroya Portillo, Lucas
dc.contributor.authorRivera, Belén
dc.contributor.authorVitale, Valentina
dc.contributor.authorJuan, Lucia de
dc.contributor.authorCruz López, Fátima
dc.contributor.otherUCH. Departamento de Medicina y Cirugía Animal
dc.contributor.otherProducción Científica UCH 2023
dc.date.accessioned2024-01-25T10:06:55Z
dc.date.available2024-01-25T10:06:55Z
dc.date.issued2023-09-12
dc.description.abstractBackground: Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak. Objectives: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS. Study design: Descriptive study of natural infection. Methods: Urine and whole blood/NS samples were collected at different time points during the hospitalisation of 21 horses involved in two EHV-1 myeloencephalopathy outbreaks in 2021 and 2023 in Spain. Quantitative real-time PCR was performed to compare the viral DNA load between BC-urine samples in 2021 and NS-urine samples in 2023. Sex, age, breed, presence of neurological signs, EHV-1 vaccination status and treatment data were recorded for all horses. Results: A total of 18 hospitalised horses during the 2021 and 2023 outbreaks were positive for EHV-1, and viral DNA was detected in urine samples from a total of 11 horses in both outbreaks. Compared with BC samples, DNA presence was detected in urine samples for longer duration and with slightly higher concentration; however, compared with NS, detection of EHV-1 in urine was similar in duration with lower DNA concentrations. Main limitations: Limited sample size, different sampling times and protocols (BC vs. NS) in two natural infection outbreak settings. Conclusions: EHV-1 was detected in the urine from naturally infected horses. Urine should be considered as complimentary to blood and NS in diagnosis of EHV-1 infection.es_ES
dc.identifier.citationVelloso Alvarez, A., Jose-Cunilleras, E., Dorrego-Rodriguez, A., Santiago-Llorente, I., de la Cuesta-Torrado, M., Troya-Portillo, L., Rivera, B., Vitale, V., de Juan, L. & Cruz-Lopez, F. (2023). Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy. Equine Veterinary Journal, Online ahead of print (12 sep.). DOI: https://doi.org/10.1111/evj.14007es_ES
dc.identifier.doihttps://doi.org/10.1111/evj.14007
dc.identifier.issn0425-1644
dc.identifier.issn2042-3306 (Electrónico)
dc.identifier.urihttp://hdl.handle.net/10637/15110
dc.language.isoenes_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relation.ispartofEquine Veterinary Journal
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectCaballoses_ES
dc.subjectHorseses_ES
dc.subjectVeterinariaes_ES
dc.subjectVeterinary medicinees_ES
dc.subjectMedicina preventivaes_ES
dc.subjectPreventive medicinees_ES
dc.subjectEnfermedad transmisiblees_ES
dc.subjectInfectious diseaseses_ES
dc.subjectAparato respiratorioes_ES
dc.subjectRespiratory systemses_ES
dc.titleDetection of equine herpesvirus-1 (EHV-1) in urine samplesduring outbreaks of equine herpesvirus myeloencephalopathyes_ES
dc.typeArtículoes_ES
dspace.entity.typePublicationes
relation.isAuthorOfPublication57a77d9e-aaa8-48a4-8205-2b98f1034acc
relation.isAuthorOfPublicationf1e1c07b-bce7-4802-bfd7-36d02170ea42
relation.isAuthorOfPublication.latestForDiscovery57a77d9e-aaa8-48a4-8205-2b98f1034acc

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Detection_Velloso_EVJ_2023.pdf
Size:
425.11 KB
Format:
Adobe Portable Document Format
Download

Collections

Dpto. Medicina y Cirugía Animal
x

The user has the obligation to use the services and content provided by the University, in particular, printed and electronic resources, in accordance with current legislation and the principles of good faith and generally accepted uses, without contravening with its performance the public order, especially, in the case in which, for the adequate performance of its activity, it needs to reproduce, distribute, communicate and/or make available, fragments of printed works or capable of being in analog or digital format, either on paper or electronic support. Law 23/2006, of July 7, which modifies the revised text of the Intellectual Property Law, approved by Royal Legislative Decree 1/1996, of April 12. B.O.E. n 162, of July 8, and concordant regulations.

@ 2024 Fundación Universitaria San Pablo CEU (Madrid). All rights reserved.

DSpace Software Copyright © 2002-2013 Duraspace