A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020

dc.centroUniversidad Cardenal Herrera-CEU
dc.contributor.authorBallester Llobell, Beatriz
dc.contributor.authorGucciardi, Francesca
dc.contributor.authorSheth Shah, Chirag Chandrakant
dc.contributor.authorRubio, Vicente
dc.contributor.authorVeses Jiménez, Verónica
dc.contributor.authorLastra, Antonio
dc.contributor.authorGonzález Pastor, Andrea
dc.contributor.authorMacaluso, Giusi
dc.contributor.authorPurpari, Giuseppa
dc.contributor.authorGuercio, Annalisa
dc.contributor.authorLorenzo Bermejo, Teresa
dc.contributor.authorMaiques Fernández, Elisa
dc.contributor.authorPadilla Blanco, Miguel
dc.contributor.authorRubio Guerri, Consuelo
dc.contributor.authorPascual Ortiz, Marina
dc.contributor.otherUCH. Departamento de Ciencias Biomédicas
dc.contributor.otherUCH. Departamento de Farmacia
dc.contributor.otherUCH. Departamento de Medicina y Cirugía
dc.contributor.otherProducción Científica UCH 2023
dc.date.accessioned2024-01-24T11:11:51Z
dc.date.available2024-01-24T11:11:51Z
dc.date.issued2023-01-26
dc.description.abstractTo investigate the influence of geographic constrains to mobility on SARS-CoV-2 circulation before the advent of vaccination, we recently characterized the occurrence in Sicily of viral lineages in the second pandemic wave (September to December 2020). Our data revealed wide prevalence of the then widespread through Europe B.1.177 variant, although some viral samples could not be classified with the limited Sanger sequencing tools used. A particularly interesting sample could not be fitted to a major variant then circulating in Europe and has been subjected here to full genome sequencing in an attempt to clarify its origin, lineage and relations with the seven full genome sequences deposited for that period in Sicily, hoping to provide clues on viral evolution. The obtained genome is unique (not present in databases). It hosts 20 single-base substitutions relative to the original Wuhan-Hu-1 sequence, 8 of them synonymous and the other 12 encoding 11 amino acid substitutions, all of them already reported one by one. They include four highly prevalent substitutions, NSP12:P323L, S:D614G, and N:R203K/G204R; the much less prevalent S:G181V, ORF3a:G49V and N:R209I changes; and the very rare mutations NSP3:L761I, NSP6:S106F, NSP8:S41F and NSP14:Y447H. GISAID labeled this genome as B.1.1 lineage, a lineage that appeared early on in the pandemic. Phylogenetic analysis also confirmed this lineage diagnosis. Comparison with the seven genome sequences deposited in late 2020 from Sicily revealed branching leading to B.1.177 in one branch and to Alpha in the other branch, and suggested a local origin for the S:G118V mutation.es_ES
dc.identifier.citationPadilla-Blanco, M., Gucciardi, F., Rubio, V., Lastra, A., Lorenzo, T., Ballester, B., González-Pastor, A., Veses, V., Macaluso, G., Sheth, C.C., Pascual-Ortiz, M., Maiques, E., Rubio-Guerri, C., Purpari, G. & Guercio, A. (2023). A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020. Frontiers in Public Health, vol. 11, art. 1098965 (26 jan.). DOI: https://doi.org/10.3389/fpubh.2023.1098965es_ES
dc.identifier.doihttps://doi.org/10.3389/fpubh.2023.1098965
dc.identifier.issn2296-2565 (Electrónico)
dc.identifier.urihttp://hdl.handle.net/10637/15087
dc.language.isoenes_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.ispartofFrontiers in Public Health, vol. 11
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectVirus
dc.subjectViruses
dc.subjectGenoma
dc.subjectGenome
dc.subjectSARS-CoV-2 (Virus)
dc.subjectItalia
dc.subjectItaly
dc.titleA SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020es_ES
dc.typeArtículoes_ES
dspace.entity.typePublicationes
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