2. Universidad Cardenal Herrera-CEU

Research on medical and veterinary entomology (MVE) is especially needed in the Caribbean islands, which are threatened by the burden of several arthropod species due to their tropical climate and environment. Identifying relevant MVE-related scientific activity in these countries and examining the characteristics of its outputs can aid in the analysis of trends, knowledge gaps and decision-making in vector-borne disease research management. This study used bibliometric methods to understand how research in this discipline developed in the insular Caribbean countries during the first two decades of this century. Data were extracted from the Web of Science Core Collection™ and SciELO Citation Index™. During the study period, the region’s scientific MVE production grew at a steady rate. Most studies focused on the order Diptera and the family Culicidae. Cuba is the largest regional producer. The predominance and growing trend of international scientific collaboration were observed. These scientific results are scattered and mainly published in journals in the United States and European countries. Recent MVE studies in the insular Caribbean have been promising; it is crucial to ensure knowledge transfer across generations of researchers to generate trained human resources that will allow these studies to continue in each country in the future.

In Guatemala, three million people are at risk of contracting cutaneous leishmaniosis, primarily in the northern regions. Despite this alarming epidemiological situation, few faunistic studies of phlebotomine sand flies have been conducted in this country, which is the most populous in Central America. An entomological survey of phlebotomine sand flies was carried out from March to September, 2018 in rural, mountainous, and forested areas of six northern Guatemalan departments with active transmission of human leishmaniosis: Petén, Huehuetenango, Quiché, Alta Verapaz, Izabal, and El Progreso. CDC miniature light traps were deployed in and around homes with recent cases of leishmaniosis in children under 10 years of age. A total of 2,605 specimens of sand flies were captured and identified, belonging to three subtribes, nine genera, and 14 species (13 of which were able to be determined), with a balanced sex ratio of 0.95 males per each female. Lutzomyia longipalpis was the most abundant species in the survey, accounting for 62.61% of the total catches; however, this species was restricted to El Progreso and Huehuetenango, with Huehuetenango having the highest capture percentage (55.54%). This vector was predominantly found outdoors, mostly in extra-domicile areas (62.29%). The male/female ratio for this species was 1.94. This study, and the entomological data we present, can aid in enhancing vector control interventions in these northern departments, thereby decreasing the incidence of leishmaniosis in humans.

Nematocera are a suborder of Diptera which historically influenced human history more than any other arthropod group. Four families show a hematophagous behaviour, one being solenophagous (Culicidae), feeding directly on blood vessels, and three being telmophagous (Ceratopogonidae, Psychodidae, and Simuliidae), feeding on blood which pools at the site where their mouthparts have formed a laceration. Although mosquitoes rank first in importance, the telmophagous are also of great interest. Objectives: to update the status of these nematocerans through a transdisciplinary approach, reviewing the main characteristics of each family, the situation of the main vector-borne diseases transmitted by them in the country, especially during the 21st century, and the most relevant species or species groups from a medical and veterinary perspective. Methods: a literature search of databases was conducted and supplemented by browsing specialized journals and citation searching. Results were reviewed and filtered. Results: to date, 84 species of biting midges (Ceratopogonidae), 13 species of sand flies (Psychodidae), and 53 species of black flies (Simuliidae) have been reported in Spain. Culicoides imicola and the Obsoletus complex stand out as the most important biting midges, as they are incriminated in the transmission of bluetongue and Schmallemberg virus; Phlebotomus perniciosus and Phlebotomus ariasi are the vectors of Leishmania infantum; and Simulium erythrocephalum and the Ornatum complex cause the greater part of nuisance and bites to humans in Spain. Conclusions: there is a need to increase research capacity in Spain in order to address several health challenges arising from the presence of telmophagous Nematocera in particular, and of blood-sucking atropods in general.

Phlebotomine sand flies (Diptera: Phlebotominae) are the principal vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae). In Central Europe, Phlebotomus mascittii is the predominant species, but largely understudied. To better understand factors driving its current distribution, we infer patterns of genetic diversity by testing for signals of population expansion based on two mitochondrial genes and model current and past climate and habitat suitability for seven post-glacial maximum periods, taking 19 climatic variables into account. Consequently, we elucidate their connections by environmental-geographical network analysis. Most analyzed populations share a main haplotype tracing back to a single glacial maximum refuge area on the Mediterranean coasts of South France, which is supported by network analysis. The rapid range expansion of Ph. mascittii likely started in the early mid-Holocene epoch until today and its spread possibly followed two routes. The first one was through northern France to Germany and then Belgium, and the second across the Ligurian coast through present-day Slovenia to Austria, toward the northern Balkans. Here we present a combined approach to reveal glacial refugia and post-glacial spread of Ph. mascittii and observed discrepancies between the modelled and the current known distribution might reveal yet overlooked populations and potential further spread.