2. Universidad Cardenal Herrera-CEU

In Guatemala, three million people are at risk of contracting cutaneous leishmaniosis, primarily in the northern regions. Despite this alarming epidemiological situation, few faunistic studies of phlebotomine sand flies have been conducted in this country, which is the most populous in Central America. An entomological survey of phlebotomine sand flies was carried out from March to September, 2018 in rural, mountainous, and forested areas of six northern Guatemalan departments with active transmission of human leishmaniosis: Petén, Huehuetenango, Quiché, Alta Verapaz, Izabal, and El Progreso. CDC miniature light traps were deployed in and around homes with recent cases of leishmaniosis in children under 10 years of age. A total of 2,605 specimens of sand flies were captured and identified, belonging to three subtribes, nine genera, and 14 species (13 of which were able to be determined), with a balanced sex ratio of 0.95 males per each female. Lutzomyia longipalpis was the most abundant species in the survey, accounting for 62.61% of the total catches; however, this species was restricted to El Progreso and Huehuetenango, with Huehuetenango having the highest capture percentage (55.54%). This vector was predominantly found outdoors, mostly in extra-domicile areas (62.29%). The male/female ratio for this species was 1.94. This study, and the entomological data we present, can aid in enhancing vector control interventions in these northern departments, thereby decreasing the incidence of leishmaniosis in humans.

Leishmaniosis is considered the most important neglected tropical disease caused by protozoan parasites in Southern European countries such as Spain. Its main etiological agent is Leishmania infantum while Phlebotomus perniciosus and Phlebotomus ariasi are the main vectors in the country. During the first half of July 2021, an entomological survey was conducted in a dog shelter and its surroundings in the municipality of Toledo (Castilla-La Mancha). Sand fly sticky traps, made of A5-size white paper coated with castor oil, were placed in six different environments inside a 200 m (the mean flight distance of Phlebotomus sp.) radius from the animal shelter. Sticky traps were set for eleven consecutive trapping nights in each site. A total of 356 phlebotomine sand flies belonging to four species in two genera were identified. These species and their abundance in the traps were: Sergentomyia minuta (70.2%), Phlebotomus perniciosus (25.9%), Phlebotomus sergenti (2.8%), and Phlebotomus papatasi (1.1%). The following list gives a breakdown of the abundance of phlebotomine sand flies found in various locations: a burrow (45.1%), a maintenance warehouse (21.3%), a holm oak´s tree hole (15.6%), an abandoned cattle barnyard (10.3%), a dog kennels area (4.3%), and surroundings of a feral feline colony (3.3%). Phlebotomus papatasi, an anthropophagic species, was only found in crevices of the maintenance warehouse. Although dogs are considered the main Leishmania reservoir, this preliminary research shows the effect of different micro-environments in the surroundings of animal shelters on sand fly abundance and diversity, and the importance that other reservoirs could play as most documented captures were associated with glires (orders Rodentia and Lagomorpha) rather than dogs and cats.