1. Investigación

Nose work with military working dogs (MWDs) in warmer-than-usual areas has led us to look for new tools to reduce both heat stress and the risk of heat stroke. One of the different strategies to manage heat stress is the use of cooling vests, such as those used in humans. The aim was to assess three cooling conditions (using two different cooling vests during exercise and the non-use of such garments) by measuring core body temperature, systemic blood pressure and pulse rate before and after the exercise (moment: four measurement times) in military dogs of the I Military Police Battalion (in Valencia, Spain). All dogs were evaluated under all three conditions during the three days of the study. Significant differences were observed between condition, moment, and the interaction of these two factors, in relation to core body temperature and pulse rate. Therefore, the use of an evaporative cooling vest may further be useful as a routine thermal control and conditioning measure in MWDs.

Background: Internet-based interventions are a promising strategy for promoting healthy lifestyle behaviors. These have a tremendous potential for delivering electronic health interventions in scalable and cost-effective ways. There is strong evidence that the use of these programs can lead to weight loss and can lower patients’ average blood pressure (BP) levels. So far, few studies have investigated the effects of internet-based programs on patients who are obese with hypertension (HTN). Objective: The aim of this study is to investigate the short- and long-term efficacy, in terms of body composition and BP parameters, of a self-administered internet-based intervention involving different modules and learning techniques aimed at promoting lifestyle changes (both physical activity and healthy eating) in patients who are obese with HTN. Methods: A randomized wait-list controlled trial design was used. We recruited 105 adults with HTN who were overweight or obese and randomly assigned them to either a 3-month internet-based intervention group (n=55) or the wait-list control group (n=50). We assessed BMI (primary outcome), body fat mass (BFM), systolic (S)BP and diastolic (D)BP, blood glucose and insulin levels, physical activity levels, and functional capacity for aerobic exercise at Time 0 (preintervention) and Time 1 (postintervention). All the patients in the wait-list control group subsequently received the intervention, and a secondary within-group analysis, which also included these participants, was conducted at Time 2 (12-month follow-up). Results: A 2-way mixed analysis of covariance showed a significant decrease in BMI, BFM, and blood glucose at 3 months in the internet-based intervention group; the effect size for the BMI and BFM parameters was moderate to large, and there was also a borderline significant trend for DBP and insulin. These results were either maintained or improved upon at Time 2 and showed significant changes for BMI (mean difference −0.4, 95% CI −0.1 to −0.6; P=.005), BFM (mean difference −2.4, 95% CI −1.1 to −3.6; P<.001), DBP (mean difference −1.8, 95% CI −0.2 to −3.3; P=.03), and blood glucose (mean difference −2, 95% CI 0 to −4; P=.04). Conclusions: Implementation of our self-administered internet-based intervention, which involved different learning techniques aimed to promote lifestyle changes, resulted in positive short- and long-term health benefits in patients who are obese with HTN.

Background: Infertility is estimated to affect 15% of couples of reproductive age. Weight management problems (being obese or overweight) are among the problems that produce infertility, both in women seeking spontaneous pregnancy and in those undergoing assisted reproduction techniques. Over the last few decades, the prevalence of obesity has increased alarmingly in our society and is now considered one of the most important public health problems. The combination of diet and exercise to achieve weight loss are currently considered an e ective intervention for the improvement of reproductive parameters in overweight or obese infertile women. In other population groups, it has been shown that Internet-based interventions are just as e ective as traditional ones, and these cover a larger population with a good cost–benefit ratio. However, to the best of our knowledge, no studies so far have analysed any specific online interventions for this group of infertile women. Thus, the objective of this project will be to evaluate the e ectiveness of an online program to promote a healthy lifestyle among women who are overweight or obese who also have a diagnosis of infertility and are on the waiting list for in vitro fertilisation treatment. Methods: This will be a randomised controlled clinical trial conducted in 94 women which will compare a self-administered Internet-based intervention promoting a healthy lifestyle in terms of diet and exercise (n = 47) to a control group that will receive standard medical care. The online program will comprise nine modules, will last for 3 months, and will be monitored every 3 months after the intervention until the final follow-up at 12 months. The main outcome will be the spontaneous pregnancy rate. Secondary outcomes will include changes in body composition, dietary and physical exercise habits, glycaemic profiles, lipid profiles, hormonal profiles, and patient quality of life related to their fertility problems. The data analysis will be done on an intention-to-treat basis. Discussion: The aim of this study is to increase our knowledge of the e ectiveness of online interventions specifically adapted to infertile women who are overweight or obese in the promotion of healthy lifestyles.

It has been suggested that physical exercise in chronic hemodialysis could improve dialysis dose and reduce postdialysis rebound. A randomized controlled trial was performed to compare a group of patients doing physical exercise during the first 2 h of hemodialysis sessions and another group doing physical exercise at home. The descriptive variables, dialysis doses measured by KtV, and rebounds (urea, creatinine, potassium, phosphorus), were recorded. For 69 patients: the mean KtV was 1.84; the 30-min rebound of creatinine was 32.37%, urea 24.39%, potassium 15.31%, and phosphorus 51.29%. For each patient, the basal measurement was compared with those determined when performing physical exercise; no statistically significant differences were observed between the changes determined to dose and rebound in the group with hemodialysis exercise compared to those of the home exercise group. In conclusion, performing physical exercise during the first 2 hours of hemodialysis sessions neither lowered postdialysis molecules rebound nor improved dialysis dose.

The aim of this study was to determine the extent to which poor physical functioning, low participation in physical activity and muscle atrophy observed among patients on hemodialysis are evident in the earlier stages of chronic kidney disease (CKD). We enrolled adults in three groups: no CKD, stage 3-4 CKD, and hemodialysis. Outcomes measured were physical activity, muscle size, thigh muscle strength, physical performance, and self-reported physical function. Patients with CKD had muscle area intermediate between the no CKD and hemodialysis groups, but they had low levels of physical activity that were similar to the hemodialysis group. Physical activity and muscle size were significantly associated with all outcomes. Kidney function was not significantly associated with muscle strength or physical performance after adjustment for physical activity and muscle size. In conclusion, interventions aimed to increase muscle mass and energy expenditure might have an impact on improving physical function of CKD patients.

El estilo de vida es un concepto complejo que incluye los aspectos externos a nosotros mismos que pueden modular e influir en nuestra salud. Los conocimientos sobre la relación entre el estilo de vida y el riesgo cardiovascular están lejos de proporcionar los niveles de evidencia que se han conseguido con los ensayos clínicos con fármacos, debido a que los estudios son escasos, fundamentalmente de tipo observacional y en grandes cohortes, con la dificultad añnadida de la no existencia de métodos seguros para conocer con precisión la ingesta diaria o lo largo de tiempo, así como la dificultad en la recogida de datos y en la medición de la adherencia debido a diferencias en la composición de los alimentos en distintas épocas y a la conducta alimentaria cambiante que posee el ser humano a lo largo del tiempo. En este documento nos hemos propuesto llevar a cabo una revisión actualizada y jerarquizada en base a las evidencias actuales, prestando atención a tres aspectos que tienen gran importancia patogénica y que podemos modificar directamente: la actividad física, el consumo de tabaco y el patrón de alimentación. Con ello pretendemos actualizar los nuevos conocimientos sobre su relación con el riesgo cardiovascular aportando evidencias útiles, constituyendo una herramienta sencilla dirigida especialmente a todos los profesionales de la salud implicados en el cuidado de personas con riesgo cardiovascular para trasladarlos a la práctica clínica, definiendo unas líneas de actuación sencillas y fáciles para ser transmitidas a las personas que reciban un consejo para la prevención primaria y secundaria de las enfermedades cardiovasculares. / Lifestyle is a complex concept that includes aspects external to ourselves that can modulate and influence our health. The knowledge of the relationship between lifestyle and cardiovascular risk does not attain the level of evidence achieved with clinical trials with drugs, because clinical studies are scarce and mainly of observational nature, albeit based on large cohorts. Nutritional epidemiology has the added difficulty of being based mostly on subjective dietary recall methods to ascertain nutrient and food intake over time, with the additional problems of incomplete data collection, variable measurements of adherence due to seasonal and geographical differences in food composition, and the changing eating behavior that human beings have over time. The purpose of this document is to carry out an updated and hierarchical review of the relationship between lifestyle and cardiovascular disease based on current evidence, paying attention to three aspects that are of great pathogenic importance and are directly modifiable: physical activity, tobacco consumption, and diet. With this, we intend to update the knowledge on this relationship, construct evidence-based recommendations, and provide a simple tool for clinical practice especially directed to health professionals involved in the care of people at cardiovascular risk, defining simple and easy strategies for individuals who receive advice for the primary and secondary prevention of cardiovascular diseases.

Introduction: interpretation of accelerometer-derived physical activity in preschool children is confounded by differences in cut-off points. Aim: the purpose of this study was to analyze physical activity in 2-to-7-year-old children to establish reference values for daily activity. Methods: observational study in children aged 2-7 years, without chronic diseases and whose parents provided informed consent. The main variable was physical activity, measured continuously over 120 hours (three workdays and two weekend days) by accelerometer. Secondary variables were weight status (body mass index [BMI] Z-score) and gender. The relationship between the main variable and secondary variables was determined through the t-test, ANOVA and the Pearson correlation coefficient. A multivariate model was used to obtain the standard deviation (SD) of all possible combinations of values, constructing percentiles of normality (x ± SD and x ± 2·SD). Results: one hundred and thirty-six children (35% of municipality children) were included in the study (54.4% of them were girls). Their weight status distribution was: 25 underweight (18.4%), 54 normal weight (39.7%), 12 risk of overweight (8.8%), 22 overweight (16.2%) and 23 obese (16.8%). The median age was 5.7 years and the mean physical activity was 592 counts/minute. The boys undertook more physical activity (p = 0.031) and the underweight and normal-weight children undertook more physical activity than the overweight and obese children (p = 0.012). There were no significant differences according to age. The multivariate analysis showed significant differences (p < 0.001) according to gender and weight status. In boys, physical activity decreased as weight status increased. In contrast, the girls in the extreme BMI groups obtained higher levels of physical activity. Conclusion: overweight and obese preschool children had lower levels of physical activity than normal weight children. Physical activity levels were higher in boys. / Introducción: la interpretación de la actividad física medida mediante actimetría en preescolares es confusa debido a los diferentes puntos de corte. Objetivo: el objetivo de este estudio fue analizar la actividad física en niños de dos a siete años para establecer valores de actividad física diaria. Método: estudio observacional en niños de dos a siete años, sin enfermedades crónicas y cuyos padres hubieran firmado el consentimiento informado. La variable principal fue la actividad física, medida durante 120 horas ininterrumpidas (tres días laborables y dos días festivos) mediante actímetros. Las variables secundarias fueron el estado de peso (puntaje z de índice de masa corporal [IMC]) y el sexo. La relación entre la variable principal y las variables secundarias fue determinada mediante el test-t, ANOVA y el coeficiente de correlación de Pearson. Se utilizó un modelo multivariable para obtener estándares de desviación en todas las posibles combinaciones de valores, construyendo percentiles de normalidad (x ± DE y x ± 2·DE). Resultados: participaron en el estudio 136 niños (35% de los niños del municipio); el 54,4% fueron chicas. La distribución de estado de peso fue: 25 niños con bajo peso (18,4%), 54 normopeso (39,7%), 12 en riesgo de sobrepeso (8,8%), 22 con sobrepeso (16,2%) y 23 con obesidad (16,8%). La media de edad fue 5,7 años y de actividad física, 592 cuentas/minuto. Los chicos realizaron mayor actividad física (p = 0,031) y los niños con bajo peso y normopeso realizaron mayor actividad física que los niños con sobrepeso y obesidad (p = 0,012). No hubo diferencias significativas respecto a la edad. El análisis multivariable mostró diferencias significativas (p < 0,001) respecto al sexo y el estado de peso. En los niños, la actividad física decreció cuando aumentó el peso. Por el contrario, las niñas en grupos de IMC extremos obtuvieron mayores niveles de actividad física. Conclusión: los preescolares en situación de sobrepeso y obesidad presentaron niveles menores de actividad física que los niños normopeso. Los niveles de actividad física fueron mayores en los chicos.

Una dosis oral de glucosa (2 g/Kg de peso) produjo cambios similares en la glucosa sanguinea de ratas gestantes de 20 dias y virgenes mientras que el aumento en la insulina plasmatica fue mayor en los animales gestantes, indicando resistencia a la insulina. En el presente trabajo se estudia si un ejercicio moderado y aerobio durante la gestación modifica la respuesta a la insulina en la madre. Ratas virgenes y preñadas de 20 días corrieron en una cinta rodante inclinada 10° durante 5 dias/semana a 20 ml mine incremento progresivo hasta 75 min en el dia 20 de ejercicio y/o gestación en que fueron sometidas a un test de tolerancia intravenoso de insulina con 10 IU de insulina porcina/Kg peso. El efecto hipoglucemiante de la insulina intravenosa mostró una mayor respuesta a la insulina en las ratas preñadas ejercitadas con respecto a las no ejercitadas, mientras que no se observa efecto en las ratas virgenes.

Free radicals generated as byproducts of normal metabolism can damage biologically relevant molecules. When their generation is increased, damage can also be increased, resulting in the development of many pathological cinditions. Antioxidant defenses protect the body from the detrimental effects of free radicals. Dietary fruits and vegetables provide a reasonable amount of compounds that act as physiological antioxidants. Although existing knowledge does not allow a final and conclusive assessment of the relevance of antioxidants for health, is does aspects of antioxidant supplementation in health and disease.