1. Investigación

The effect of mild stress on various plasma metabolites in the rat has been studied. Mild stress resulted in significant decreases in liver size and glycogen content, as well as in an increase of blood glucose. In addition, plasma lactate, insulin, glycerol and urea, as well as a number of amino acids were altered by stress. These data indicate that minimal stress can have major effects upon the composition of blood, and suggest the need for strict rrecautions on the handling of animals during blood sampling. The site of blood extraction - tail tip i•s. neck - was also found to have a significant effect on plasma lactate. glucosi! and urea concentrations. In stressed animals the differences between tail- and neck blood composition were increased.

A radiochemical method based on dansylation of plasma samples with Dansyl-chloride and thin layer chromatography on polyamide sheets is presented for the quantitative individual determination of plasma amino acids in very small blood samples. The results are first corrected with a norvaline internal standard and secondly with ~pecific recovery factors for each amino acid. The results agree closely with other methods and with already published plasma normal amino acids values in healthy adult rats.

In this work is described a simplified plasma deproteinizatior. procedure using acetone and very small amounts of plasma. The precipitation is carried out in small-bore capillary tubes, and acetone is used as deproteinizing agent. This method can be advantageously compared with heat, Somogyi's, perchloric, and even trichloroacetic deproteinization procedures for its good amino acid preservation and protein removal capabilities.

A method for the determination of the total muscle mass in small experimental animals is presented. Muscle proteins were quantified in the 1 M LiCl-soluble and distilled water-insoluble fraction of the eyeless, brainless, eviscerated and skinned carcass, as compared with a striated muscle sample from the same animal used as standard and processed in the same way as the whole carcass. The non-muscular tissues left in the carcass do not interfere with the final results. The total muscle mass in adult rats was estimated as 42.0 ± 2.8 % of the ln vivo weight.

Se discuten las expresiones de actividad enzimatica mas utilizadas en estudios comparados de actividad en diferentes tejidos: microkatales por unidad de peso del tejido, por unidad de peso de proteina y por unidad de peso de AON. Se utiliza tambien la expresi6n de microkatales presentes en un 6rgano determinado referidos a unidad de peso del animal, 100 g en el caso de la rata. Las diversas expresiones se han aplicado a los niveles de aspartato transaminasa, glutamato deshidrogenasa y AMP desaminasa en higado, musculo estriado de pata trasera y riiiones de rata adulta. De Ios datos presentados se concluye que las mediciones de actividades enzimaticas en tejidos deben ser expresadas en mas de una forma, ya que la informaci6n obtenida a partir de una sola de ellas puede ser substancialmente distinta de la obtenida con otra de ellas, dando lugar a posibles conclusiones err6neas de! papel metab6lico jugado por el enzima en un tejido determinado.

Plasma amino acid concentrations, together with other metabolic parameters were determined in thyroidectomized rats treated with daily injections of sal ins (hypothyroid), and 250 ,ug/kg L-T4 {hypothyroid). Data were compared with sham-operated controls. TherP. is a genera! incre'!SE> in plasma amino acid concentrations in hyperthyroidism, a limited in· crease only in several amino acid r.oncentrations in hypothyroid rats as compared with controls, and a considerable difference between the plasma aminograms of both groups. Amino acid homeostasis seems to be subject to greater modification in hyperthyroidism than in hypothyroidism.

The effects of food deprivation for up to 24 hours on plasma metabolic parameters in the rat have been studied. Liver dry weight and glycogen content dropped significantly from a hours of food deprivation onwards. Total muscle glycogen supplied about as much glycosyl residues or precursors as did the liver. Plasma glucose, urea, lactate and total and essential amino acids decreased significantly from 3 hours of fasting onwards. Glycerol, free fatty acids, beta-hydroxybutyrate and acetoacetate showed significant increases with fasting. Alanine, serine, arginine, threonine, aspartate plus asparagine and proline showed significant decreases with fasting. Several other amino acids showed almost nc change with fasting. Lysine, leucine plus isoleucine and taurine showed biphasic changes in their concentrations with a minimum at 6 hours and a transient recovery at 12 hours of fasting. Essential amino acids decreased more than the non essential ones. With fasting there is a shift in ammonia disposal with lower urea concentrations as nitrogen is better conserved. The results seem to suggest that there i~ a constant release of substrates, through liver and peripheral tissue proteolysis, that is counteracted by differential utilization of amino acids during fasting.

The effect of ethyl ether, pentobarbital sodium (Nembutal®) and chloral hydrate anesthesia on the concentrations of glucose, lactate, glycerol, insulin, individual amino acids and urea have been studied in rat plasma, as well as the glycogen content in the animal liver and striated muscle. Control animals were injected with saline solution. Two samples were taken from each animal at 15 and 30 minutes from the beginning of anesthesia. These samples were obtained from the cut tail's tip and from the neck wound produced by beheading the animal. Results show considerable differences between the three sets of animals and their controls as well as in their tail versus neck values. The overall effect of the three anesthetics used were roughly the same in spite of their different actions upon the different parameters studied. This was most marked in the aminograms. Significant changes induced were rather similar in number for all. The metabolite concentration profile in each situation may be used as a reference for possible artifacts induced by any experimental approach using these anesthetics.