1. Investigación

Equine ventricular repolarisation wave can be influenced by many physiological and pathological factors. T wave abnormalities have been related to a shorter time for ventricular filling, reduced stroke volume, cardiac output and exercise performance in racehorses. The present research performed electrocardiographic recordings in 14 four-year-old male Andalusian horses, when they were untrained and after three months of an aerobic training programme. Leads I, aVF, V10, V1R, V3R, V1L and V3L were used. It was aimed to assess the incidence of abnormal T waves in this breed, according to the criteria of abnormalities established for other equine breeds, to evaluate if the changes induced by training in the T wave are the same that those reported in the athletic heart syndrome in canine and human athletes and, to analyse if the abnormal T waves could have been related to changes in the plasma concentrations of Na, K, and Cl, since these electrolytes are involved in the cardiac electrical processes. It was found out that the incidence of abnormal T waves was quite high in the Andalusian breed, especially in the precordial leads. Moreover, the incidence of abnormal T waves increased in precordial leads and decreased in lead I and aVF after training. The abnormal T waves after training were shorter and had the same voltage, changes which were different to those presented for the athlete’ heart syndrome. The horses with abnormal T waves had higher plasma K concentrations, both before and after training. Plasma Na and Cl concentration at rest decreased after training. Plasma Na concentrations were positively related to T wave duration and negatively to T wave voltage.

Plasma K accumulation during exercise results from the balance between exchange through biological membranes (mainly muscle fibres and erythrocytes), distribution to other tissues and the haemoconcentration. In the present study, the effect of exercise and training on plasma K concentrations and its relationships with other physiological variables have been analysed in two equine breeds. Twenty male Andalusian (AN) and ten Angloarabian (AA) horses, 7 females and 3 males, were subjected to two standardised exercise tests, composed of four workloads, before and after training. Heart rate (HR) was monitored and venous blood was withdrawn at rest, before each exercise level and during recovery. The following parameters were analysed: packed cell volume (PCV), plasma K, lactate (LA) and total protein (TPP). Furthermore, the horses were filmed and three kinematic parameters were studied: stride duration (SD), frequency (SF) and length (SL). Exercise induced an increase in K from 6 and 8 m/sec in AA and AN horses respectively, a steady-state until the end of the exercise and a decrease after 2 min of recuperation. Some interbreed differences existed, with higher K levels in the AN horses, due to the higher relative exercise intensity, stride frequency and haemoconcentration. K was correlated with HR, PCV, TPP, SL, SD and SF. Training caused a decrease in K in AN, but not in AA horses. Plasma K seems to be a good indicator of the physical effort intensity, fitness and training degrees, but it was not related to the magnitude of the glycolytic response to exercise.

El objetivo de este trabajo ha sido describir un sistema de pun-tuación objetivo, a partir de índices de funcionalidad cardiovas-cular y metabólica, que permita la discriminación entre caba-llos según su potencial atlético. Se han estudiado 45 caballosadultos, Pura Raza Española (PRE), machos. Tras un calenta-miento al trote (4 m/s), los animales realizaron un test de ejer-cicio, a velocidades de 5, 6,7y8m/s, cubriendo 1.000 m encada carga de esfuerzo. Se monitoreó la frecuencia cardiaca(FC) y se extrajeron muestras de sangre venosa en reposo,tras el calentamiento, después de cada fase del test y a los 2,4, 6, 8, 10, 15 y 30 minutos de una recuperación activa. Ensangre entera, se determinó la concentración de hemoglobina(HB) y el valor hematócrito (HTO). En plasma, se midieron losniveles de lactato (LA). Los índices de funcionalidad oxidativoshan sido: HBo’ y HTOo’ (HB y HTO a 8 m/s), V150 y VLA2 (ve-locidad a 150 lpm y 2 mmol/L de LA respectivamente), FCLA2(FC a 2 mmol/L de LA) y LA150 (LA a 150 lpm). Como índicesglicolíticos-mixtos, se han considerado: FCmáx (FC máxima),FCo’ (FC a 8 m/s), LAmáx (LA máximo), V200 y VLA4 (veloci-dades a 200 lpm y 4 mmol/L de LA), FCLA4 (FC a 4 mmol/Lde LA) y LA200 (LA a 200 lpm). Los índices que mejor han dis-criminado a los caballos PRE según su nivel de forma físicahan sido VLA2, LA150 y HTOo’ (oxidativos) y FCo’, VLA4 yV200 (glicolíticos-mixtos). VLA2, VLA4 y V200 estuvieron co-rrelacionados positivamente con el potencial físico y FCo’,HTOo’ y LA150, de modo negativo.

Achieving the successful recovery of sled dogs is one of the key tasks for veterinary teams involved in clinical care for middle-distance sled dog competitions. This study compares the efficacy of capacitive-resistive electrical transfer (CRet) with that of massage in the treatment of lower back pain in 40 short-haired sled dogs during a medium-distance snow sled race (LekkarodTM-2021). The dogs were divided into two groups: a CRet group (20 dogs) and a massage group (20 dogs). All subjects received a single 18 min treatment session and were evaluated one hour after the end of the treatment. A multivariate analysis of variance (MANOVA) was performed in which pre- and post-treatment pain measures were evaluated in relation to age and type of treatment. Older dogs were found to have higher significant pain scores before starting treatment. Both treatments reduce pain short-term in all cases. However, post-treatment pain values were significantly lower in dogs treated with CRet when compared to dogs treated with massage. The results show that capacitiveresistive electrical transfer has better short-term results and is beneficial in both younger and older dogs, making this technique attractive to veterinary teams working in canine sporting competitions.

Nose work with military working dogs (MWDs) in warmer-than-usual areas has led us to look for new tools to reduce both heat stress and the risk of heat stroke. One of the different strategies to manage heat stress is the use of cooling vests, such as those used in humans. The aim was to assess three cooling conditions (using two different cooling vests during exercise and the non-use of such garments) by measuring core body temperature, systemic blood pressure and pulse rate before and after the exercise (moment: four measurement times) in military dogs of the I Military Police Battalion (in Valencia, Spain). All dogs were evaluated under all three conditions during the three days of the study. Significant differences were observed between condition, moment, and the interaction of these two factors, in relation to core body temperature and pulse rate. Therefore, the use of an evaporative cooling vest may further be useful as a routine thermal control and conditioning measure in MWDs.

Bikejöring is a type of dryland mushing requiring high-intensity aerobic effort, with speed peaks close to 42 km/h. Greysters (crosses between the German Shorthaired Pointer and the Greyhound) often participate in such events and perform well. The objective of this comparative study was to evaluate the clinical use of non-invasive methods in assessing the cardiovascular health of 22 Greyster dogs in physical training, by determining the differences between different cardiovascular parameters before and after physical training. Blood pressure, heart rate and echocardiographic results were compared. The mean age of the dogs was 4.4 years ff. 1.8% and 54.5% were female. All participating dogs regularly participated in bikejöring. Post-exercise increases were observed in systolic blood pressure (SBP), mean arterial pressure (MBP) and pulse pressure (SBPD), with diastolic blood pressure (DBP) remaining stable. Changes of clinical interest were observed in numerous echocardiographic variables such as left ventricle fractional shortening (LVFS), left ventricule ejection fraction (LVEF), E-point to septal separation (EPSS), cardiac output (CO), cardiac index (CI), posterior wall thickness at end-diastole (PWd) and major/minor axis ratio (MA/ma), including a decrease in the shortening fraction and an increase in EPSS after exercise. These clinical findings were observed in both males and females; they do not appear to be associated with dilated cardiomyopathy, but rather with a cardiovascular response to physical training. This study derives from the real interest of clinical veterinarians who care for highly trained canine athletes. It contributes to an increase in knowledge of the di erent cardiac adaptations of such dogs after intense exercise and serves to differentiate these from pathologic conditions.