Browsing by Author "Alonso Sousa, Santiago"

Las proteínas de fase aguda (PFAs), como el amiloide sérico tipo A (AAS), la haptoglobina (Hp) y la proteína C reactiva (CRP) son marcadores específicos de inflamación, proporcionando un método alternativo de diagnóstico en la monitorización del estado sanitario del caballo. El análisis de dichas proteínas durante la gestación podría ayudar a detectar posibles complicaciones patológicas, establecer un tratamiento y monitorizar la respuesta al mismo. El objetivo de este estudio ha sido determinar las concentraciones séricas de AAS, Hp y CRP junto con el leucograma, para verificar si la gestación fisiológicamente normal manifiesta una respuesta de fase aguda (RFA), y por tanto, evaluar el seguimiento de la misma en yeguas PRE. Las concentraciones de Hp se modificaron significativamente durante la segunda mitad de la gestación, sin modificaciones en el resto de parámetros analizados. En conclusión, la Hp podría ser un marcador útil en el seguimiento de gestaciones fisiológicamente normales en yeguas PRE. Acute phase proteins like serum amyloid A (SAA), haptoglobin (Hp) and C reactive protein (CRP) are specific inflammation biomarkers who can provide a diagnostic alternative system for the healthy state evaluation in the horse. Their analysis of these proteins during the pregnancy could help to detect possible pathologic complications, establish a treatment and evaluate their response. The objective of the current study was determined the serum concentrations of AAS, Hp and CRP and the white blood cells concentration as well trying to verify if the physiological normal pregnancy cause an acute phase response, and therefore, following the pregnancy process in Spanish Purebred mares. Hp concentrations changed significantly during the second half of pregnancy, without changes in the rest of analytic parameters. In conclusion, Hp could be a useful mark in the physiological normal pregnancy evaluation in Spanish Purebred mares.

Mediterranean mussel (Mytilus galloprovincialis) is a species of bivalve mollusk that has a high capacity in filtering the water nearby and concentrate the virus and bacteria present in it. This can constitute a microbiological risk when they are consumed afterwards. Thus, depending on water pollution, mussels suffer a depuration process, usually controlled using Escherichia coli as indicator. The aim of this study was to determine the microbiological quality of mussels from various sources after purifying, and to consider the utility of the use of Clostridium perfringens as quality indicator. For this, microbiological analysis were performed in fifteen lots of mussel samples obtained from Spain and Italy. Aerobic mesophilic microorganisms, Enterobacteriaceae, E. coli, Vibrio spp., Listeria monocytogenes, Cl. perfringens, and the presence Salmonella spp. were measured. The results showed the presence of Salmonella spp. in four batches of mussels from various locations and Cl. perfringrens in thirteen of them. All samples were within the legal limits for E. coli. In conclusion, the results propose that the use of E. coli as an indicator microorganism for the mussels' depuration process is not sufficient to assure the microbiological quality of the product. Thus, it would be appropriate to include of Cl. perfringens, as an additional indicator, in the control carried out during and after the refinement is done. Finally, the results emphasized the necessity to properly cook this food product prior to consumption, as it allows the destruction of any microorganisms present.