Carceller Zazo, María CarmenGil Herrero, María LuisaAlcaraz Tormo, María JoséGuillén Salazar, IsabelProducción Científica UCH 2021UCH. Departamento de Farmacia2022-04-142022-04-142021-01-29Carceller, M.C., Guillén, M.I., Gil, M.L. & Alcaraz, M.J. (2021). Extracellular vesicles do not mediate the anti-inflammatory actions of mouse-derived adipose tissue mesenchymal stem cells secretome. International Journal of Molecular Sciences, vol. 22, i. 3 (29 jan.), art. 1375. DOI: https://doi.org/10.3390/ijms220313751422-0067 (Electrónico)http://hdl.handle.net/10637/13660Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1422-0067/22/3/1375Este artículo pertenece al número especial "The Role of Mesenchymal Stem Cells on Inflammatory and Fibrotic Diseases".Adipose tissue represents an abundant source of mesenchymal stem cells (MSC) for therapeutic purposes. Previous studies have demonstrated the anti-inflammatory potential of adipose tissue-derived MSC (ASC). Extracellular vesicles (EV) present in the conditioned medium (CM) have been shown to mediate the cytoprotective effects of human ASC secretome. Nevertheless, the role of EV in the anti-inflammatory effects of mouse-derived ASC is not known. The current study has investigated the influence of mouse-derived ASC CM and its fractions on the response of mouse-derived peritoneal macrophages against lipopolysaccharide (LPS). CM and its soluble fraction reduced the release of pro-inflammatory cytokines, adenosine triphosphate and nitric oxide in stimulated cells. They also enhanced the migration of neutrophils or monocytes, in the absence or presence of LPS, respectively, which is likely related to the presence of chemokines, and reduced the phagocytic response. The anti-inflammatory effect of CM may be dependent on the regulation of toll-like receptor 4 expression and nuclear factor- B activation. Our results demonstrate the antiinflammatory effects of mouse-derived ASC secretome in mouse-derived peritoneal macrophages stimulated with LPS and show that they are not mediated by EV.application/pdfenopen accessCélulas madre - Uso terapéutico.Stem cells - Therapeutic use.Adipose tissues - Therapeutic use.Tejido adiposo - Uso terapéutico.Inflammation - Treatment.Macrófagos.Macrophages.Inflamación - Tratamiento.Artículohttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es