Mateo Jiménez, Eva MaríaSoria López, José MiguelTonino, Rik Paulus BernardusCantó Catalá, AntolínMonroy Noyola, AntonioGarcía Esparza, María ÁngelesMiranda Sanz, MaríaUCH. Departamento de Ciencias BiomédicasUCH. Departamento de FarmaciaProducción Científica UCH 20222022-11-092022-11-092022-09-06Mateo, E., Tonino, R. P. B., Canto, A., Monroy Noyola, A., Miranda, M., Soria, J. M., & Garcia Esparza, M. A. (2022). The neurotoxic effect of Ochratoxin-A on the hippocampal neurogenic niche of adult mouse brain. Toxins, vol. 14, i. 9, art. 624 (06 sep.). DOI: http://dx.doi.org/10.3390/toxins140906242072-6651 (Electrónico)http://hdl.handle.net/10637/14029Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://www.mdpi.com/2072-6651/14/9/624Este artículo de investigación pertenece al número especial titulado "Evaluation and Prevention of Mycotoxin Contamination and Toxicological Effects".Ochratoxin A (OTA) is a common secondary metabolite of Aspergillus ochraceus, A. carbonarius, and Penicillium verrucosum. This mycotoxin is largely present as a contaminant in several cereal crops and human foodstuffs, including grapes, corn, nuts, and figs, among others. Preclinical studies have reported the involvement of OTA in metabolic, physiologic, and immunologic disturbances as well as in carcinogenesis. More recently, it has also been suggested that OTA may impair hippocampal neurogenesis in vivo and that this might be associated with learning and memory deficits. Furthermore, aside from its widely proven toxicity in tissues other than the brain, there is reason to believe that OTA contributes to neurodegenerative disorders. Thus, in this present in vivo study, we investigated this possibility by intraperitoneally (i.p.) administering 3.5 mg OTA/kg body weight to adult male mice to assess whether chronic exposure to this mycotoxin negatively affects cell viability in the dentate gyrus of the hippocampus. Immunohistochemistry assays showed that doses of 3.5 mg/kg caused a significant and dose-dependent reduction in repetitive cell division and branching (from 12% to 62%). Moreover, the number of countable astrocytes (p < 0.001), young neurons (p < 0.001), and mature neurons (p < 0.001) negatively correlated with the number of i.p. OTA injections administered (one, two, three, or six repeated doses). Our results show that OTA induced adverse effects in the hippocampus cells of adult mice brain tissue when administered in cumulative doses.application/pdfenopen accessCélulas - Morfología.Cells - Morphology.Hipocampo (Cerebro) - Efectos de los productos químicos.Ocratoxina A - Efectos fisiológicos.Hippocampus (Brain) - Effects of chemicals on.Micotoxinas - Efectos fisiológicos.Mycotoxins - Physiological effect.Neurotoxicología.Neurotoxicology.Ochratoxin A - Physiological effect.Artículohttps://doi.org/10.3390/toxins14090624https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es