Roiban, Gheorghe-DoruReetz, Manfred T.Agudo Torres, RubénUniversidad San Pablo-CEU. Departamento de Ciencias Farmacéuticas y de la SaludGrupo: Parasitología e Inmunología molecular con aplicación biotecnológica, diagnóstica y terapéutica (PARINM)2024-04-102024-04-102013-05-06Gheorghe-Doru Roiban, Rubén Agudo, Manfred T. Reetz, Stereo- and regioselectivity in the P450-catalyzed oxidative tandem difunctionalization of 1-methylcyclohexene, Tetrahedron, Volume 69, Issue 26, 2013, Pages 5306-5311, ISSN 0040-4020, https://doi.org/10.1016/j.tet.2013.04.132.0040-4020http://hdl.handle.net/10637/15722Acceso al contenido del artículo en la siguiente URL: https://doi.org/10.1016/j.tet.2013.04.132The selective partial oxidation of small non-functionalized molecules using biocatalysis based on P450 monooxygenases is known to be difficult due to the expected poor regio- and stereoselectivity, but in this study it was nevertheless attempted. 1-Methylcyclohexene was subjected to oxygen-mediated bio catalytic oxidation using P450-BM3 as the catalyst. Both oxidative hydroxylation and epoxidation were observed, in some cases leading to hydroxy epoxides with high diastereo- and enantioselectivity espe cially when employing BM3 mutants.enopen accessEnantioselectivityCH-activationP450MonooxygenaseDiastereoselectivityArtículo10.1016/j.tet.2013.04.132https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es