Gutiérrez Albanchez, EnriqueGarcía Villaraco, AnaHorche Trueba, IgnacioRamos Solano, BeatrizGutiérrez Mañero, Francisco JavierLucas García, José AntonioUniversidad San Pablo-CEU. Facultad de FarmaciaGrupo: Biotecnología de la Interacción Planta-Microbioma (PLANTA-MICROBIOMA)2024-11-182024-11-182024-11Peribacillus aracenensis sp.nov., a plant growth promoting bacteria for agriculture in water-scarce conditions isolated from Pinus pinaster rhizosphere., HELIYON, https:// doi.org/10.1016/j.heliyon.2024.e399732405-8440http://hdl.handle.net/10637/16446Información complementaria de los datos de investigación de este estudio se pueden encontrar en https://www.ncbi.nlm.nih.gov/bioproject/?term=bbb004A gram-positive, nonpathogenic, central endospore-forming, flagellated strain, was successfully isolated from the rhizosphere of Pinus pinaster in Aracena (Spain). Its optimal growth conditions are 28 ◦C, pH 6, and 0 % salinity. It is able to assimilate glucose, L-fucose, L-arabinose, b-metil-Dxylose and shows high catabolic capacity. The major fatty acids (>79.20 % of the total fatty acids) are anteiso C15:0 > iso C15:0 > C14:0. A phylogenetic analysis based on the 16S rRNA gene sequence revealed similarity to P. frigoritolerans DSM8801T (99.9 %), P. castrilensis CECT30509T (99.8 %), and P. simplex DSM1321T (99.6 %). Comparison of whole genomes revealed that strain BBB004T is more similar to P. simplex DSM1321T. According to ANI (93.54 %), AAI (94 %), dDDH (60.6), %G + C (0.12), TETRA (0.99822) and intergenomic distance (0.2835) values, therefore this species is different to the closest. A total of 133 genes unique to Peribacillus BBB004T were identified. Supported by these analyses, strain BBB004T (=LMG32742T = CCUG76477T) was proposed as the type strain for a new species, named “Peribacillus aracenensis sp. nov.”application/pdfenopen accessNew specieWater stressInduced systemic toleranceAdaptative metabolismBlueberryArtículo10.1016/j.heliyon.2024.e39973https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es