Fernández Pastor, SandraSilvestre Castelló, María DoloresSilvestre Castelló, DoloresLópez Mendoza, María CarmenProducción Científica UCH 2021UCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los AlimentosUCH. Departamento de Farmacia2024-10-152024-10-152021-02Fernández-Pastor, S., Silvestre Castelló, D., & López-Mendoza, M. C. (2021). Stability of the antimicrobial capacity of human milk against "Cronobacter Sakazakii" during handling. Journal of Human Lactation, 37(1), 139–146. https://doi.org/10.1177/08903344209325740890-33441552-5732 (Electrónico)http://hdl.handle.net/10637/16280Este es el pre-print del siguiente artículo: Fernández-Pastor, S., Silvestre Castelló, D., & López-Mendoza, M. C. (2021). Stability of the antimicrobial capacity of human milk against "Cronobacter Sakazakii" during handling. Journal of Human Lactation, 37(1), 139–146, que se ha publicado de forma definitiva en https://doi.org/10.1177/0890334420932574This is the pre-peer reviewed version of the following article: Fernández-Pastor, S., Silvestre Castelló, D., & López-Mendoza, M. C. (2021). Stability of the antimicrobial capacity of human milk against "Cronobacter Sakazakii" during handling. Journal of Human Lactation, 37(1), 139–146. Copyright © 2021. DOI: https://doi.org/10.1177/0890334420932574Este artículo es la versión preprint, siguiendo la política de acceso de la editorial Sage.Background: Neonatal infections with Cronobacter sakazakii have recently been associated with the consumption of expressed human milk. Study Aims: (1) To evaluate whether human milk has antimicrobial capacity against C. sakazakii and (2) to determine the stability of its capacity when it is subjected to various treatments. Methods: The antimicrobial capacity of human milk against C. sakazakii was evaluated using an observational, cross-sectional, comparative design. Mature human milk samples (N = 29) were subjected to different treatments. After incubation at 37°C for 72 hr, samples were compared with fresh milk on the stability of their antimicrobial capacity. Two-way analysis of variance (ANOVA) was performed. Results: In fresh milk, counts of C. sakazakii were reduced by 47.26% (SD = 6.74) compared to controls. In treated milk, reductions were: refrigeration at 4°C for 72 hr (M = 33.84, SD = 13.84), freezing at –20°C for 1, 2, and 3 months (M = 40.31, SD = 9.10; M = 35.96, SD = 9.39; M = 26.20, SD = 13.55, respectively), Holder pasteurization (M = 23.56, SD = 15.61), and human milk bank treatment with (M = 14.37, SD = 18.02) and without bovine fortifier (M = 3.70, SD = 23.83). There were significant differences (p < .05) between fresh and treated milk. Conclusions: Human milk has antimicrobial capacity against C. sakazakii. However, its capacity is negatively influenced by common preservation and hygienization methods. Milk should be stored refrigerated for a maximum of 72 hr or frozen for a short period of time.enopen accessLactancia maternaBreast feedingLeche maternaMother's milkCronobacter sakazakiiArtículohttps://doi.org/10.1177/0890334420932574https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es