Humphrey, SuzanneMillán, Álvaro SánToll Riera, MacarenaConnolly, JohnFlor Duro, AlejandraChen, JohnÚbeda Morant, CarlesMacLean, R. CraigPenadés Casanova, José RafaelProducción Científica UCH 2021UCH. Departamento de Ciencias Biomédicas2022-04-292022-04-292021-10-06Humphrey, S., San Millán, Á., Toll-Riera, M., Connolly, J., Flor-Duro, A., Chen, J., Ubeda, C., MacLean, R.C., & Penadés, J.R. (2021). Staphylococcal phages and pathogenicity islands drive plasmid evolution. Nature Communications, vol. 12, art. 5845 (06 oct.). DOI: https://doi.org/10.1038/s41467-021-26101-52041-1723 (Electrónico)http://hdl.handle.net/10637/13679Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://www.nature.com/articles/s41467-021-26101-5.pdfConjugation has classically been considered the main mechanism driving plasmid transfer in nature. Yet bacteria frequently carry so-called non-transmissible plasmids, raising questions about how these plasmids spread. Interestingly, the size of many mobilisable and nontransmissible plasmids coincides with the average size of phages (~40 kb) or that of a family of pathogenicity islands, the phage-inducible chromosomal islands (PICIs, ~11 kb). Here, we show that phages and PICIs from Staphylococcus aureus can mediate intra- and inter-species plasmid transfer via generalised transduction, potentially contributing to non-transmissible plasmid spread in nature. Further, staphylococcal PICIs enhance plasmid packaging efficiency, and phages and PICIs exert selective pressures on plasmids via the physical capacity of their capsids, explaining the bimodal size distribution observed for non-conjugative plasmids. Our results highlight that transducing agents (phages, PICIs) have important roles in bacterial plasmid evolution and, potentially, in antimicrobial resistance transmission.application/pdfenopen accessBacterias patógenas.Pathogenic bacteria.Bacterial genetic.Evolutionary genetics.Bacteriophages.Genética bacteriana.Genética evolutiva.Bacteriófagos.Artículohttps://doi.org/10.1038/s41467-021-26101-5https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es