Madrigal Verdú, PilarBallester Lurbe, BegoñaTerrado Vicente, JoséMoreno Bravo, Juan AntonioPuelles, E.Jurado Sánchez, SandraGarcía Verdugo, José ManuelGómez Roda, OlgaPérez Roger, IgnacioProducción Científica UCH 2022UCH. Departamento de Medicina y Cirugía AnimalUCH. Departamento de Ciencias Biomédicas2021-11-232021-11-232022-04-01Madrigal, M.P., Ballester-Lurbe, B., Gómez, O., Moreno-Bravo, J.A., Puelles, E., Jurado, S. et al. (2022). Rnd3 is necessary for the correct oligodendrocyte differentiation and myelination in the central nervous system. Brain Structure and Function, vol. 227, i. 3 (01 apr.), pp. 829-841. DOI: https://doi.org/10.1007/s00429-021-02419-01863-2653.1863-2661 (Electrónico)http://hdl.handle.net/10637/13197Este artículo se encuentra disponible en la siguiente URL: https://link.springer.com/article/10.1007/s00429-021-02419-0This is a post-peer-review, pre-copyedit version of an article published in Madrigal, MP., Ballester-Lurbe, B., Gómez, O., Moreno-Bravo, JA., Puelles, E., Jurado, S., Garcia-Verdugo, JM., Pérez-Roger, I. & Terrado, J. (2022). Rnd3 is necessary for the correct oligodendrocyte differentiation and myelination in the central nervous system. Brain Structure and Function, vol. 227, i. 3 (01 apr.), pp. 829-841. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00429-021-02419-0.Este es el post-print del siguiente artículo: Madrigal, MP., Ballester-Lurbe, B., Gómez, O., Moreno-Bravo, JA., Puelles, E., Jurado, S., Garcia-Verdugo, JM., Pérez-Roger, I. & Terrado, J. (2022). Rnd3 is necessary for the correct oligodendrocyte differentiation and myelination in the central nervous system. Brain Structure and Function, vol. 227, i. 3 (01 apr.), pp. 829-841, que se ha publicado de forma definitiva en http://dx.doi.org/10.1007/s00429-021-02419-0Rho small GTPases are proteins with key roles in the development of the central nervous system. Rnd proteins are a subfamily of Rho GTPases characterized by their constitutive activity. Rnd3/RhoE is a member of this subfamily ubiquitously expressed in the CNS, whose specific functions during brain development are still not well defined. Since other Rho proteins have been linked to the myelination process, we study here the expression and function of Rnd3 in oligodendrocyte development. We have found that Rnd3 is expressed in a subset of oligodendrocyte precursor cells and of mature oligodendrocytes both in vivo and in vitro. We have analyzed the role of Rnd3 in myelination using mice lacking Rnd3 expression (Rnd3gt/gt mice), showing that these mice exhibit hypomyelination in the brain and a reduction in the number of mature and total oligodendrocytes in the corpus callosum and striatum. The mutants display a decreased expression of several myelin proteins and a reduction in the number of myelinated axons. In addition, myelinated axons exhibit thinner myelin sheaths. In vitro experiments using Rnd3gt/gt mutant mice showed that the differentiation of the precursor cells is altered in the absence of Rnd3 expression, suggesting that Rnd3 is directly required for the differentiation of oligodendrocytes and, in consequence, for the correct myelination of the CNS. This work shows Rnd3 as a new protein involved in oligodendrocyte maturation, opening new avenues to further study the function of Rnd3 in the development of the central nervous system and its possible involvement in demyelinating diseases.application/pdfesopen accessSistema nervioso central.Central nervous system.Proteínas.Myelin sheath.Células.Cells.Mielina.Proteins.Artículohttps://doi.org/10.1007/s00429-021-02419-0https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es