Barbas Arribas, CoralCastro, MarioViana Arribas, MartaBonet Serra, BartoloméHerrera Castillón, Emilio2011-09-192011-09-191997http://hdl.handle.net/10637/1017En: Journal of chromatography A, ISSN 0021-9673 1997. n. 778 : 415-420 p.A high-performance liquid chromatographic (HPLC) method to determine vitamins A, Ap and E simultaneously was developed with direct extraction of vitamins from rat tissues with n-hexane and probe sonicating. The dry residue was redissolved in chloroform-methanol. Vitamins A and Ap were detected by UV-Vis and vitamin Eby fluorescence. Vitamin K, used as internal standard, was detected both by UV-Vis and by fluorescence. Standards and samples were checked for linearity giving correlation coefficients that were higher than 0.99 in the concentration range of 3.1-9.4 for vitamin A, 8.2-24.7 for vitamin Ap and 0.6-1.7 nmol/g in the case of liver extracts and 0.5-3.0 nmol/g in the case of placenta. The intra-assay precision (R.S.D.) varied between 1.48 and 7.25, whereas inter-assay precision was between 4.99 and 7.03. Recoveries ranged between 94±8 and 107±5%. Results from the application of this method to different rat tissues having wide range of vitamin content are presented.application/pdfenVitaminsArtículohttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.eshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es