Dea Ayuela, María AuxiliadoraGaliana Roselló, CristinaLalatsa, KaterinaSerrano López, Dolores RemediosProducción Científica UCH 2018UCH. Departamento de Farmacia2019-11-202019-11-202018-11-20Dea Ayuela, MA., Galiana-Roselló, C., Lalatsa, A. & Serrano, DR. (2018). Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs). Current Topics in Medicinal Chemistry, vol. 18, n. 16, pp. 1358-1374. DOI: https://doi.org/10.2174/15680266186661810250957351568-02661873-4294 (Electrónico)http://hdl.handle.net/10637/10692Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: http://www.eurekaselect.com/166607/articleThis is the pre-peer reviewed version of the following article: Dea Ayuela, MA., Galiana-Roselló, C., Lalatsa, A. & Serrano, DR. (2018). Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs). Current Topics in Medicinal Chemistry, vol. 18, n. 16, pp. 1358-1374, which has been published in final form at https://doi.org/10.2174/1568026618666181025095735Este es el post-print del siguiente artículo: Dea Ayuela, MA., Galiana-Roselló, C., Lalatsa, A. & Serrano, DR. (2018). Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs). Current Topics in Medicinal Chemistry, vol. 18, n. 16, pp. 1358-1374, que se ha publicado de forma definitiva en https://doi.org/10.2174/1568026618666181025095735One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector-borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on loopmediated isothermal amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point- of-care diagnostic tests.application/pdfesopen accessQuímica farmacéutica.Pharmaceutical chemistry.Bioquímica clínica.Chagas, Enfermedad de - Diagnóstico.Clinical biochemistry.Malaria - Diagnosis.Leishmaniasis - Diagnosis.Paludismo - Diagnóstico.Trypanosomiasis - Diagnosis.Tripanosomiasis - Diagnóstico.Leishmaniasis - Diagnóstico.Chagas' disease - Diagnosis.Artículohttps://doi.org/10.2174/1568026618666181025095735https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es