Vega Martín, ElenaSomoza Hernández, BeatrizGonzález Blázquez, RaquelViana Arribas, MartaManzano Lista, Francisco J.Martín Ramos, MiriamGarcía Prieto, Concepción F.Rubio, Miguel A.Calle Pascual, Alfonso L.Lionetti, LillàFernández Alfonso, María SoledadGil Ortega, MartaAlcalá Díaz-Mor, MartínUniversidad San Pablo-CEU. Facultad de Farmacia. Departamento de Química y BioquímicaGrupo de Metabolismo y Función Vascular (MET-VASC)2023-07-052023-07-052020-01-28Vega-Martín E, González-Blázquez R, Manzano-Lista FJ, Martín-Ramos M, García-Prieto CF, Viana M, Rubio MA, Calle-Pascual AL, Lionetti L, Somoza B, Fernández-Alfonso MS, Alcalá M, Gil-Ortega M. Impact of caloric restriction on AMPK and endoplasmic reticulum stress in peripheral tissues and circulating peripheral blood mononuclear cells from Zucker rats. J Nutr Biochem. 2020 Apr;78:108342. doi: 10.1016/j.jnutbio.2020.108342. Epub 2020 Jan 9.http://hdl.handle.net/10637/14500The activation of endoplasmic reticulum (ER) stress and a reduction of AMP-dependent protein kinase (AMPK) phosphorylation have been described in obesity. We hypothesize that a moderate caloric restriction (CR) might contribute to reducing ER stress and increasing AMPK phosphorylation in peripheral tissues from genetically obese Zucker fa/fa rats and in peripheral blood mononuclear cells (PBMCs). Zucker Lean and Zucker fa/fa rats were fed with chow diet either ad libitum (AL) (C, as controls) or 80% of AL (CR) for 2 weeks, giving rise to four experimental groups: Lean C, Lean CR, fa/fa C and fa/fa CR. CR significantly increased AMPK phosphorylation in the liver, perirenal adipose tissue (PRAT) and PBMCs from fa/fa rats but not in the subcutaneous AT (SCAT), suggesting a reduced response of SCAT to CR. Liver samples of fa/fa rats exhibited an increased mRNA expression of PERK, EIF-2α, XBP-1(s), Chop and caspase 3, which was significantly reduced by CR. PRAT exhibited an overexpression of Edem and PDIA-4 in fa/fa rats, but only PDIA-4 expression was reduced by CR. eIF-2α phosphorylationwas significantly increased in all studied tissues fromfa/fa rats and reduced by CR. A negative correlationwas detected between p-AMPK and p-eIF-2α in the liver, PRAT and PBMCs from fa/fa rats but not in SCAT. This study shows that a moderate CR reduces ER stress and improves AMPK phosphorylation in several peripheral tissues and in circulating PBMCs, suggesting that alterations observed in PBMCs could reflect metabolic alterations associated with obesity. © 2020 Elsevier Inc. All rights reserved.application/pdfenopen accessCaloric restrictionEndoplasmic reticulum stressObesityCirculating peripheral blood mononuclear cellsArtículo10.1016/j.jnutbio.2020.108342https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es