Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/724

Dual effect of glucose on LDL oxidation: dependence on vitamin E.

Título : Dual effect of glucose on LDL oxidation: dependence on vitamin E.
Autor : Otero Gómez, Paola
Herrera Castillón, Emilio.
Bonet Serra, Bartolomé
Materias: Low density lipoprotein.Glucose.Vitamin E.Free radicals.
Resumen : The aim of the present study was to determine the direct effect of glucose on LDL oxidation, a key step in the development of atherosclerosis. Purified human LDL were incubated with glucose (500 mg/di) and LDL oxidation was started by adding CuCl2 to the media. Glucose delayed the vitamin E consumption, but accelerated the formation of conjugated dienes and increased both the formation of thiobarbituric acid reacting substances (TBARS) and LDL electrophoretic mobility. When LDL were incubated with increasing concentrations of glucose and submitted to oxidation, the formation of conjugated dienes, TBARS, and the electrophoretic mobility increased in a concentrationdependent manner. When LDL was enriched with vitamin E, it showed a delay in the formation of conjugated dienes, even in the presence of glucose. To determine whether glucose had any effect on LDL oxidation, once the process was started and vitamin E consumed, LDL were submitted to oxidation and, at different times thereafter, glucose was added into the media. Under these conditions glucose also accelerated the LDL oxidation. In summary, present results show that in LDL submitted to oxidation, glucose delays the early phases of the oxidation, slowing the vitamin E consumption, but it accelerates the rate of LDL oxidation once LDL vitamin E has been consumed; the effect being concentration-dependent.
Descripción : En: Free radical biology and medicine. 2002. n. 33 : 1133-1140 p. 0891-5849
URI : http://hdl.handle.net/10637/724
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Fecha de publicación : 19-sep-2002
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.